A novel Schiff base cobalt(III) complex induces a synergistic effect on cervical cancer cells by arresting early apoptosis stage

A new schiff base cobalt(III) complex [ N , N ′-bis(2′-hydroxyphenylacetone)-o-ethanediamine] cobalt(III) ( M3 ) has been synthesized and characterized by single X-ray crystallography. The cytotoxicity of complex M3 was evaluated against HeLa, LoVo, A549, A549/cis cancer cell lines, and the normal c...

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Bibliographic Details
Published in:Biometals 2021-04, Vol.34 (2), p.277-289
Main Authors: Liao, Wen-Hui, Song, Xue-Qing, Kong, Yan-Jie, Bao, Rui-Dan, Li, Fang-Fang, Zhou, Jie, Zhao, Qi-Hua, Xu, Jing-Yuan, Xie, Ni, Xie, Ming-Jin
Format: Article
Language:English
Subjects:
Apoptosis
Biochemistry
Biomedical and Life Sciences
Biotechnology
c-Myc protein
Cell Biology
Cell cycle
Cell proliferation
Cervical cancer
Cervix
Cisplatin
Cobalt
Crystallography
Cytotoxicity
Deoxyribonucleic acid
DNA
DNA biosynthesis
DNA damage
Imines
Life Sciences
Medicine/Public Health
Microbiology
Myc protein
Nuclear division
Pharmacology/Toxicology
Plant Physiology
Platinum
Proteasomes
Protein C
Synergistic effect
Toxicity
Tumor cell lines
X-ray crystallography
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Summary:A new schiff base cobalt(III) complex [ N , N ′-bis(2′-hydroxyphenylacetone)-o-ethanediamine] cobalt(III) ( M3 ) has been synthesized and characterized by single X-ray crystallography. The cytotoxicity of complex M3 was evaluated against HeLa, LoVo, A549, A549/cis cancer cell lines, and the normal cell lines LO2 by MTT assays. The IC 50 is in the range of 6.27-22.68 μM, which is somewhat lower than cisplatin on the basis of platinum molar concentration. Furthermore, anticancer mechanistic studies showed that the complex M3 inhibited cell proliferation by blocking DNA synthesis and then acted on nuclear division of HeLa cells over time. Moreover, western blot analysis indicated M3 dramatically decreased the target protein c-Myc and KLF5 expression levels, and activated many signaling pathways including ER stress, apoptosis, cell cycle and DNA damage in HeLa. M3 did not affect proteasomal activity.
ISSN:0966-0844
1572-8773
DOI:10.1007/s10534-020-00278-6