Integration of choline geranate into electrospun protein scaffolds affords antimicrobial activity to biomaterials used for cutaneous wound healing

Wound healing attempts to maintain homeostasis in the wound while minimizing the risk of infection to the tissue by foreign agents, such as opportunistic bacterial pathogens. Biofilms established by these pathogens are a common cause of chronic infections that slow the healing process. Preparation o...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2021-09, Vol.109 (9), p.1271-1282
Main Authors: Bardsley, Tatum A., Evans, Charlotte L., Greene, Joshua R., Audet, Robert, Harrison, Mackenzie J., Zimmerman, Maxwell, Nieto, Nathan C., Del Sesto, Rico E., Koppisch, Andrew T., Kellar, Robert S.
Format: Article
Language:English
Subjects:
Administration, Cutaneous
Anti-Infective Agents - chemistry
Anti-Infective Agents - pharmacology
Antiinfectives and antibacterials
antimicrobial
Antimicrobial activity
Antimicrobial agents
Bandages
Biocompatible Materials - chemistry
Biofilms
Biomaterials
Biomedical materials
Cages
Choline
Choline - chemistry
Choline - pharmacology
choline geranate
Cross-Linking Reagents - chemistry
Devices
Drug Liberation
Electrospinning
Fibroblasts
Fibroblasts - chemistry
Health risks
Homeostasis
Humans
Inactivation
Ionic liquids
Ionic Liquids - chemistry
ionic liquids/deep eutectic solvents
Magnetic properties
Material properties
Materials research
Materials science
Medical dressings
Microbial Sensitivity Tests
Neonates
NMR
Nuclear magnetic resonance
Opportunist infection
Pathogens
Persistent Infection - drug therapy
Proteins
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Scanning electron microscopy
Skin
Spectrometry
Tissue Engineering
Tissue Scaffolds - chemistry
Wound healing
Wound Healing - drug effects
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Summary:Wound healing attempts to maintain homeostasis in the wound while minimizing the risk of infection to the tissue by foreign agents, such as opportunistic bacterial pathogens. Biofilms established by these pathogens are a common cause of chronic infections that slow the healing process. Preparation of skin wound healing devices comprised of electrospun proteins associated with skin have been shown to accelerate the healing process relative to conventional wound dressings. In this work, we have developed electrospinning methods to incorporate the antimicrobial ionic liquid/deep eutectic solvent choline geranate (CAGE) into these devices. Integration of CAGE into the dressing material was verified via 1H nuclear magnetic resonance spectrometry, and the effect on the material property of the resultant devices were assessed using scanning electron microscopy. CAGE‐containing devices demonstrate a concentration‐dependent inactivation of exogenously applied solutions of both gram‐positive and gram‐negative pathogens (Enterococcus sp and Pseudomonas aeruginosa, respectively), but maintain their ability to serve as a compatible platform for proliferation of human dermal neonatal fibroblasts.
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.34788