Rapid quantification of insulin in human milk by immunoassay

Human milk (HM) contains numerous non-nutritive bioactive factors, amongst which the peptide hormone insulin. HM insulin has been suggested to accelerate intestinal maturation, thereby promoting feeding tolerance. Therefore, recombinant human insulin for enteral administration has been developed whi...

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Bibliographic Details
Published in:European journal of clinical nutrition 2021-07, Vol.75 (7), p.1152-1154
Main Authors: Mank, Elise, Naninck, Eva F G, van den Akker, Chris H P, Heijboer, Annemieke C
Format: Article
Language:English
Subjects:
Breast milk
Clinical trials
Freeze thaw cycles
Freeze-thawing
Health aspects
Immunoassay
Insulin
Measurement
Nutritional aspects
Pediatric research
Room temperature
Schedules
Stability analysis
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Summary:Human milk (HM) contains numerous non-nutritive bioactive factors, amongst which the peptide hormone insulin. HM insulin has been suggested to accelerate intestinal maturation, thereby promoting feeding tolerance. Therefore, recombinant human insulin for enteral administration has been developed which might serve as supplement to HM or formula for preterm infants. However, the natural course of the HM insulin concentration directly following delivery is unknown, which hampers the development of dosage schedules in clinical trials. The aim of this study was to validate a method for insulin determination in small volumes of HM, and to assess the stability of HM insulin. The results showed that the HM insulin concentration can be measured rapidly and reliably by using an automated immunoassay. In addition, HM insulin is stable at 4 °C for at least 72 h, at room temperature for a maximum of 12 h, at -20 °C for at least 2.5 years, and during at least five freeze-thaw cycles.
ISSN:0954-3007
1476-5640
DOI:10.1038/s41430-020-00832-y