NanH and PknG putative virulence factors as a recombinant subunit immunogen against Corynebacterium pseudotuberculosis infection in mice

•Mice immunized with rNanH+saponin (G2) exhibited a survival rate of 60%.•Mice immunized with rPknG+saponin (G3) exhibited a survival rate of 20%.•G2 showed the best protection against Corynebacterium pseudotuberculosis challenge.•rNanH induced significant production of IgG2a antibodies.•rNanH induc...

Full description

Saved in:
Bibliographic Details
Published in:Vaccine 2020-12, Vol.38 (51), p.8099-8106
Main Authors: Thais de Oliveira Silva, Mara, Barros de Pinho, Rodrigo, da Rocha Fonseca, Bárbara, Silvestre Brilhante Bezerra, Francisco, Severo Sabedra Sousa, Fernanda, Kommling Seixas, Fabiana, Collares, Tiago, José Meyer Nascimento, Roberto, Wagner Portela, Ricardo, Ariston Carvalho Azevedo, Vasco, Borsuk, Sibele
Format: Article
Language:English
Subjects:
Abscesses
Animal models
Animals
Antibodies
Antigens
Bacterial Vaccines
Caseous lymphadenitis
Cellular response
CLA
Corynebacterium Infections - prevention & control
Corynebacterium pseudotuberculosis
Corynebacterium pseudotuberculosis - genetics
Cytokines
E coli
Enzymes
Gene expression
Genes
Immune response
Immune response (humoral)
Immune system
Immunization
Immunoglobulin G
Immunoprophylaxis
Infections
Interferon
Interleukin 10
Interleukin 12
Interleukin 4
Kinases
Lymphadenitis
Lymphadenitis - prevention & control
Lymphocytes T
Mice
Neuraminidase H
Protein kinase G
Proteins
Pseudotuberculosis
Saline solutions
Saponins
Survival
Vaccines
Virulence
Virulence factors
Virulence Factors - genetics
γ-Interferon
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Mice immunized with rNanH+saponin (G2) exhibited a survival rate of 60%.•Mice immunized with rPknG+saponin (G3) exhibited a survival rate of 20%.•G2 showed the best protection against Corynebacterium pseudotuberculosis challenge.•rNanH induced significant production of IgG2a antibodies.•rNanH induced significant production of cytokines related to a Th1 immune response. Despite the economic and zoonotic relevance of caseous lymphadenitis, a competent immunoprophylaxis tool is still necessary. Here, we evaluated two putative virulence factors of Corynebacterium pseudotuberculosis, rNanH, and rPknG, as recombinant subunit vaccines in a murine model against the infection by C. pseudotuberculosis. Three groups of ten Balb/c mice each were inoculated with a sterile 0.9% saline solution (G1), rNanH (G2), or rPknG (G3) in formulations containing saponin as an adjuvant. The mice received two vaccine doses intercalated by a 21-day interval and were challenged with 2 × 104 CFU/mL of the C. pseudotuberculosis MIC–6 strain 21 days after the last immunization. The total IgG, IgG1, and IgG2a production levels increased significantly in the experimental groups (G2 and G3) on day 42. The highest levels of IgG2a antibodies in G2 and G3 were observed compared to IgG1 levels. G3 showed a significant (p 
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2020.11.010