Catalytic Amplification of Electrochemical Signal in Homogeneous Solution Using an Entropy-driven DNA Circuit

The electrochemical signal from ferrocene on a DNA probe was successfully modulated in a homogeneous solution by the template-directed formation and dissociation of an inclusion complex with β-cyclodextrin on another probe. The electrochemical response was amplified by combining with a DNA circuit,...

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Bibliographic Details
Published in:Analytical Sciences 2021/03/10, Vol.37(3), pp.533-537
Main Authors: KITAMURA, Yusuke, YOSHIMURA, Keisuke, KURAMOTO, Ryo, KATSUDA, Yousuke, IHARA, Toshihiro
Format: Article
Language:English
Subjects:
Amplification
Analytical Chemistry
Biosensing Techniques
Catalysis
Catalysts
Chemistry
Circuits
Complementary DNA
cyclodextrin
Cyclodextrins
Deoxyribonucleic acid
DNA
DNA - analysis
DNA circuit
DNA Probes - chemistry
electrochemical biosensor
Electrochemical Techniques
Electrochemistry
Electrodes
Entropy
Entropy of solution
ferrocene
Ferrous Compounds - chemistry
homogeneous assay
inclusion complex
Inclusion complexes
Metallocenes - chemistry
Nucleic acids
signal amplification
Solutions
Surface Properties
Target detection
β-Cyclodextrin
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Summary:The electrochemical signal from ferrocene on a DNA probe was successfully modulated in a homogeneous solution by the template-directed formation and dissociation of an inclusion complex with β-cyclodextrin on another probe. The electrochemical response was amplified by combining with a DNA circuit, in which the target DNA served as a catalyst. This system did not require any modification of a complementary DNA with the ferrocene-modified probe on the electrode surface to separate the bound/free probe for the detection of 200 nM target DNA.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.20SCN04