Genome- and Mass Spectrometry-Guided Discovery of Ralstoamides A and B from Ralstonia solanacearum Species Complex

Strains of Ralstonia solanacearum species complex (RSSC) are devastating plant pathogens distributed globally with a wide host range and genetic diversity. Many RSSC strains harbor the polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) hybrid gene rmyA/rmyB for ralstonin production. We r...

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Bibliographic Details
Published in:ACS chemical biology 2020-11, Vol.15 (11), p.2860-2865
Main Authors: Komatsu, Shoko, Tsumori, Chiaki, Ohnishi, Kouhei, Kai, Kenji
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Genome, Bacterial
Lipopeptides - chemistry
Lipopeptides - genetics
Mass Spectrometry
Nicotiana - microbiology
Peptide Synthases - genetics
Polyketide Synthases - genetics
Ralstonia solanacearum - chemistry
Ralstonia solanacearum - genetics
Solanum lycopersicum - microbiology
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Summary:Strains of Ralstonia solanacearum species complex (RSSC) are devastating plant pathogens distributed globally with a wide host range and genetic diversity. Many RSSC strains harbor the polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) hybrid gene rmyA/rmyB for ralstonin production. We report that ralstoamides A (1) and B (2), which are ralstonin-like but shorter lipopeptides, were discovered from the Japanese strains using accumulated RSSC genome data and LC/MS-based metabolite analysis. Their structures, including absolute configurations, were elucidated by spectroscopic analysis and chemical techniques. ramA, a PKS-NRPS gene for ralstoamide production, was identified from the producer strains by genome sequencing and gene-deletion experiments. Based on the analysis of biosynthetic genes of ralstoamides and ralstonins, we suggest the occurrence of NRPS-module reduction of rmyA/rmyB genes in some RSSC strains. This possible molecular evolution changed not only the structures, but also the biological activity of RSSC lipopeptides.
ISSN:1554-8929
1554-8937
DOI:10.1021/acschembio.0c00605