Effect of valproic acid on the hepatic differentiation of mesenchymal stem cells in 2D and 3D microenvironments

Mesenchymal stem cells (MSCs) have multi-lineage differentiation potential which make them an excellent source for cell-based therapies. Histone modification is one of the major epigenetic regulations that play central role in stem cell differentiation. Keeping in view their ability to maintain gene...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2021-02, Vol.476 (2), p.909-919
Main Authors: Rashid, Saman, Qazi, Rida-e-Maria, Malick, Tuba Shakil, Salim, Asmat, Khan, Irfan, Ilyas, Amber, Haneef, Kanwal
Format: Article
Language:English
Subjects:
Acetylation
Analysis
Biochemistry
Biomedical and Life Sciences
Cardiology
Cell differentiation
Collagen
Differentiation (biology)
Divalproex
DNA binding proteins
Epigenetic inheritance
Epigenetics
Gene expression
Glycogen
Glycogens
Histone deacetylase
Histones
Life Sciences
Liver
Medical Biochemistry
Mesenchymal stem cells
Microenvironments
Oncology
Regeneration
Scaffolds
Stem cells
Valproic acid
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Summary:Mesenchymal stem cells (MSCs) have multi-lineage differentiation potential which make them an excellent source for cell-based therapies. Histone modification is one of the major epigenetic regulations that play central role in stem cell differentiation. Keeping in view their ability to maintain gene expression essential for successful differentiation, it was interesting to examine the effects of valproic acid (VPA), a histone deacetylase inhibitor, in the hepatic differentiation of MSCs within the 3D scaffold. MSCs were treated with the optimized concentration of VPA in the 3D collagen scaffold. Analyses of hepatic differentiation potential of treated MSCs were performed by qPCR, immunostaining and periodic acid Schiff assay. Our results demonstrate that MSCs differentiate into hepatic-like cells when treated with 5 mM VPA for 24 h. The VPA-treated MSCs have shown significant upregulation in the expression of hepatic genes, CK-18 ( P  
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-020-03955-9