Protective effects of Antrodia camphorata extract against hypoxic cell injury and ischemic stroke brain damage

Ischemic stroke is the most prevalent stroke condition in the world resulted in either a transient ischemic attack or long‐lasting neurological problems due to the interrupted or reduced blood flow to the brain. Antrodia camphorata is a well‐known medicinal mushroom native to Taiwan and is familiar...

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Bibliographic Details
Published in:Phytotherapy research 2021-03, Vol.35 (3), p.1609-1620
Main Authors: Kong, Zwe‐Ling, Hsu, Ya‐Ting, Johnson, Athira, Tsai, Tung‐Han, Miao, Song, He, Jia‐Ling, Tsou, David
Format: Article
Language:English
Subjects:
Animals
Antioxidants
anti‐inflammatory
anti‐oxidative
Antrodia camphorata
Blood flow
Brain
Brain damage
Brain injury
Brain Ischemia - drug therapy
Cell injury
Cerebral blood flow
Cobalt
Head injuries
Heme
Hypoxia
Ischemia
ischemia stroke
Ischemic Stroke - drug therapy
Male
Malondialdehyde
mRNA
Mushrooms
neuroprotective
Nitric oxide
Nitric-oxide synthase
Oxidative stress
Oxygenase
Pheochromocytoma cells
Polyporales - chemistry
Prostaglandin endoperoxide synthase
Rats
Rats, Sprague-Dawley
Reactive oxygen species
Reperfusion
Stroke
Transient ischemic attack
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Summary:Ischemic stroke is the most prevalent stroke condition in the world resulted in either a transient ischemic attack or long‐lasting neurological problems due to the interrupted or reduced blood flow to the brain. Antrodia camphorata is a well‐known medicinal mushroom native to Taiwan and is familiar due to its medicinal effects. The current study investigated the protective effect of A. camphorata‐alcohol extracts (AC‐AE) against cobalt (II) chloride (CoCl2)‐induced oxidative stress in vitro and ischemia/reperfusion‐induced brain injury in vivo. The rats were pre‐treated with AC‐AE for 4 weeks. Our results showed that AC‐AE reduced cell damage and decreased reactive oxygen species (ROS) production in C6 and PC12 cells under CoCl2‐induced hypoxic condition. AC‐AE doses (385, 770, 1,540 mg/kg/day, 4 weeks) increased nuclear factor erythroid 2–related factor 2 (Nrf2) and heme oxygenase‐1 (HO‐1) mRNA expressions and decreased inducible nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2) mRNA expressions in Sprague Dawley rat. Besides, it decreased stroke infarct size and increased the level of antioxidants in both brain and serum. Furthermore, it reduced the formation of malondialdehyde (MDA) after ischemia/reperfusion (I/R). Our results suggested that AC‐AE exerted an effective reduction of ischemia stroke by regulating ROS production.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.6928