Characterization and expression analysis of tandemly-replicated asc genes in the Japanese medaka, Oryzias latipes

ASC is a component of the inflammasome playing crucial roles in the inflammatory response. In mammals, ASC induces pyroptosis and inflammatory cytokine production. In this study, three asc genes (asc1, asc2, and asc3) from the Japanese medaka (Oryzias latipes) were identified and characterized. Thes...

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Published in:Developmental and comparative immunology 2021-02, Vol.115, p.103894-103894, Article 103894
Main Authors: Morimoto, Natsuki, Okamura, Yo, Kono, Tomoya, Sakai, Masahiro, Hikima, Jun-ichi
Format: Article
Language:English
Subjects:
Aeromonas hydrophila
Aeromonas hydrophila - immunology
Animals
Animals, Genetically Modified
ASC
Cell Line
Edwardsiella - immunology
Edwardsiella piscicida
Fish Diseases - genetics
Fish Diseases - immunology
Fish Diseases - microbiology
Fish Proteins - genetics
Fish Proteins - metabolism
Gene Duplication - immunology
Gene expression
Gene Expression Regulation - immunology
Gene Knockout Techniques
Inflammasomes - genetics
Japanese medaka (Oryzias latipes)
Oryzias - genetics
Oryzias - immunology
Oryzias - microbiology
Phylogeny
Pyroptosis
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Summary:ASC is a component of the inflammasome playing crucial roles in the inflammatory response. In mammals, ASC induces pyroptosis and inflammatory cytokine production. In this study, three asc genes (asc1, asc2, and asc3) from the Japanese medaka (Oryzias latipes) were identified and characterized. These asc genes were tandem replicates on chromosome 16, and their exon-intron structures differed between them. All three ASCs conserved the pyrin and caspase-recruitment domains, which are important for inflammasome formation. In phylogenetic analysis, all ASCs clustered with those of other teleosts. The asc1 expression levels were significantly higher in several organs than those of asc2 and asc3, suggesting that asc1 may act as a dominant asc in the Japanese medaka. Expression of the three asc genes showed different patterns during Aeromonas hydrophila and Edwardsiella piscicida infections. Furthermore, their expression was adequately down-regulated in the medaka fin-derived cells stimulated with ATP for 12 h, while asc2 expression was statistically up-regulated after nigericin stimulation for 24 h. Moreover, the expression of asc2 and asc3 was significantly higher in the skin of ASC-1-knockout medaka than in that of the wild type medaka during A. hydrophila infection. •Three asc genes were identified from Japanese medaka Cab strain.•The asc1 expression was significantly high in several organs.•The asc2 and -3 expression was significantly increased in the ASC-1-KO medaka skin.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2020.103894