Isolation, structural characterization, and biological activities of galactomannans from Rhizopogon luteolus and Ganoderma adspersum mushrooms

Polysaccharides are essential compounds that contribute to the biological activities of mushrooms. Two new galactomannans (Galactomannan I and II) were isolated from R. luteolus and G. adspersum. Their structures were characterized using FT-IR, 1D, and 2D-NMR techniques. Both isolated galactomannans...

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Bibliographic Details
Published in:International journal of biological macromolecules 2020-12, Vol.165 (Pt B), p.2395-2403
Main Authors: Tel-Çayan, Gülsen, Muhammad, Akhtar, Deveci, Ebru, Duru, Mehmet Emin, Öztürk, Mehmet
Format: Article
Language:English
Subjects:
Agaricales - chemistry
Anticholinesterase activity
Antioxidant activity
Antioxidants - pharmacology
Basidiomycota - chemistry
Characterization
Cholinesterase Inhibitors - pharmacology
Chromatography, High Pressure Liquid
Galactomannan
Ganoderma - chemistry
Ganoderma adspersum
Mannans - chemistry
Mannans - isolation & purification
Mannans - pharmacology
Molecular Conformation
Reference Standards
Rhizopogon luteolus
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Summary:Polysaccharides are essential compounds that contribute to the biological activities of mushrooms. Two new galactomannans (Galactomannan I and II) were isolated from R. luteolus and G. adspersum. Their structures were characterized using FT-IR, 1D, and 2D-NMR techniques. Both isolated galactomannans I and II mainly include D-mannose and D-galactose in the molar percentages of 0.81:1.0 and 1:1.4, respectively. The GPC calculation demonstrated that the molecular weights are about 5240 and 5090 Da, respectively. Their structures comprise of β-(1,4)-mannose (Man) backbone units with α-(1,6)-galactose (Gal) single unit as a side group. The anticholinesterase activity of galactomannans was tested against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), spectrophotometrically. Antioxidant activity was also measured by six assays (ABTS•+, DPPH•, O2•−, β-carotene-linoleic acid, metal chelating, and CUPRAC assays). Galactomannan II indicated close inhibitory activity to galantamine against AChE (61.04 ± 0.45%) and BChE (59.70 ± 1.15%) at 50 μg/mL concentration. Nevertheless, both galactomannans showed low antioxidant activity in all tests. This study reveals that mainly, Galactomannan II could be used as a new natural promising anticholinesterase agent. •Two new galactomannans were isolated from mushroom species.•Galactomannans were characterized by FT-IR, 1D and 2D NMR, MS analysis.•Galactomannans mainly included D-mannose and D-galactose.•Antioxidant and anticholinesterase activities of galactomannans were determined.•Galactomannan II indicated strong anticholinesterase activity.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2020.10.040