Identification and characteristics of insulin-like growth factor system in the brain, liver, and gonad during development of a seasonal breeding teleost, Pampus argenteus

•The IGF-1, IGF-2, IGF-3, IGF1RA, IGF1RB, and IGF2R were identified in silver pomfret.•The IGF-3 of silver pomfret was a different IGF-3 than that of other teleosts.•The IGF-1 and IGF-2 maintained high transcript levels in both sexes’ liver and gonad.•The gonadal development were related with IGFs i...

Full description

Saved in:
Bibliographic Details
Published in:General and comparative endocrinology 2021-01, Vol.300, p.113645-113645, Article 113645
Main Authors: Gu, Weiwei, Yang, Yang, Ning, Chao, Wang, Yajun, Hu, Jiabao, Zhang, Man, Kuang, Siwen, Sun, Yibo, Li, Yaya, Zhang, Youyi, Sun, Jiachu, Ying, Dongxu, Xu, Shanliang
Format: Article
Language:English
Subjects:
Gametogenesis
Gonadal development
Insulin-like growth factor
Silver pomfret
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•The IGF-1, IGF-2, IGF-3, IGF1RA, IGF1RB, and IGF2R were identified in silver pomfret.•The IGF-3 of silver pomfret was a different IGF-3 than that of other teleosts.•The IGF-1 and IGF-2 maintained high transcript levels in both sexes’ liver and gonad.•The gonadal development were related with IGFs in this pieces. Reproductive activity is closely related to the development and function of the brain and liver in teleosts, particularly in seasonal breeding teleosts. This study measured the involvement of the insulin-like growth factor (IGF) system in controlling the reproduction of the silver pomfret Pampus argenteus, a seasonal breeding tropical to temperate commercial fish. We cloned and characterized the cDNAs of igfs (igf2 and igf3) and igfrs (igf1ra, igf1rb, and igf2r) and examined their transcript levels in relation to seasonal reproduction. Phylogenetic analyses revealed that two types of IGFs (IGF-1 and IGF-2) and three types of IGFRs (IGF1RA, IGF1RB, and IGF2R) of the silver pomfret were clustered with those of teleosts; however, IGF-3 was a transmembrane protein different with the IGF-3 of other teleosts. The expression of IGF-3 was gonad-specific in the silver pomfret. The transcript levels of igf1 in the female brain were the highest, and the levels of igfrs in both sexes’ brains increased during gametogenesis. Meanwhile, igfs and igfrs maintained high transcript levels in both sexes’ liver and gonad during vitellogenesis and spermatogonia proliferation. We concluded that the development and activities of brain, liver, and gonad were related to the IGF system (IGFs and IGFRs). And the IGFs were mainly expressed in the liver. Nevertheless, gonadal development, especially vitellogenesis and spermatogonia proliferation, were related with IGFs in this species.
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2020.113645