Sustainable Lipase Production by Diutina rugosa NRRL Y-95 Through a Combined Use of Agro-Industrial Residues as Feedstock

The potential use of alternative culture media towards the development of a sustainable bioprocess to produce lipases by Diutina rugosa is clearly demonstrated. First, a synthetic medium containing glucose, peptone, yeast extract, oleic acid, and ammonium sulfate was proposed, with lipase activity o...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2021-02, Vol.193 (2), p.589-605
Main Authors: de Freitas, Maria de Fátima M., Cavalcante, Lucas S., Gudiña, Eduardo J., Silvério, Sara C., Rodrigues, Sueli, Rodrigues, Lígia R., Gonçalves, Luciana R. B.
Format: Article
Language:English
Subjects:
Agricultural wastes
Ammonium
Ammonium sulfate
Biochemistry
Bioreactors
Biotechnology
Chemistry
Chemistry and Materials Science
Cultivation
Culture media
Design of experiments
Experimental design
Food processing industry wastes
Food waste
Lipase
Molasses
Oleic acid
Peptone
Peptones
Residues
Sulfates
Sustainable development
Syrups & sweeteners
Yeasts
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Summary:The potential use of alternative culture media towards the development of a sustainable bioprocess to produce lipases by Diutina rugosa is clearly demonstrated. First, a synthetic medium containing glucose, peptone, yeast extract, oleic acid, and ammonium sulfate was proposed, with lipase activity of 143 U/L. Then, alternative culture media formulated with agro-industrial residues, such as molasses, corn steep liquor (CSL), and olive mill waste (OMW), were investigated. An experimental design was conducted, and only CSL concentration was found to have a positive effect in lipase production. The highest lipase activity (561 U/L) was produced on a mixture of molasses (5 g/L), CSL (6 g/L), OMW (0.5% v /v), 0.5 g/L of ammonium sulfate, and 3 g/L of peptone at 24 h of cultivation. Lipase production was also carried out in a 1-L bioreactor leading to a slightly higher lipase activity at 24 h of cultivation. The semi-purified enzyme exhibits an optimum temperature and pH of 40 °C and 7.0, respectively. Finally, the media cost per unit of lipase produced (UPC) was influenced by the medium components, specially by the inducer used. The lowest UPC was obtained when the agro-industrial residues were combined and used at the improved concentrations.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-020-03431-6