Influence of nutrient status on the biohydrogen and lipid productivity in Parachlorella kessleri: a biorefinery approach

The commercial reality of microalgal biotechnology for the production of individual bioactives is constrained by the high cost of production and requires a biorefinery approach. In this investigation, we examined the influence of different nutrient deprivation (nitrogen (N), phosphorus (P), sulphur...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2020-12, Vol.104 (23), p.10293-10305
Main Authors: Hamed, Seham M., Kapoore, Rahul Vijay, Raut, Mahendra P., Vaidyanathan, Seetharaman, Wright, Phillip C.
Format: Article
Language:English
Subjects:
Aerobic conditions
Algae
Anaerobic conditions
Aquatic microorganisms
Bioenergy and Biofuels
Biofuels
Biohydrogen
Biomass
Biomedical and Life Sciences
Biorefineries
Biosynthesis
Biotechnology
Cell division
Chlorophyll
Chlorophyll A
Chlorophyta
Deprivation
Fatty acid composition
Fatty acids
Green algae
Hydrogen
Hydrogen production
Life Sciences
Lipids
Manganese
Microalgae
Microbial Genetics and Genomics
Microbiology
Nitrogen
Nutrient deficiency
Nutrient status
Nutrients
Observations
Pandalus kessleri
Parachlorella kessleri
Phosphorus
Physiological aspects
Refining
Sulfur
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Summary:The commercial reality of microalgal biotechnology for the production of individual bioactives is constrained by the high cost of production and requires a biorefinery approach. In this investigation, we examined the influence of different nutrient deprivation (nitrogen (N), phosphorus (P), sulphur (S) and manganese (Mn)) on growth, chlorophyll a (Chl a ), biohydrogen (H 2 ) and fatty acid profiles in Parachlorella kessleri EMCCN 3073 under both aerobic and anaerobic conditions. Anaerobic conditions combined with the nutrient deprivation resulted in cell division blockage, reduction in Chl a and remarkable changes in pH, whereas a significant increase in the H 2 production was observed after 24 h. The highest cumulative H 2 productivity was observed in N-deficient medium (300 μL/L, day 9) followed by Mn-deficient medium (250 μL/L, day 7). The highest H 2 production rate (3.37 μL/L/h) was achieved by Mn-deficient medium after 24 h. In terms of fatty acid composition, P. kessleri exhibited a differential response to different nutrient stresses. Under aerobic conditions, N-deficient media resulted in the highest lipid content (119% compared to control, day 7), whereas earlier lipid induction at (1–3 days) was observed with Mn- and S-deficient media with 18–91% and 25–34% increase, respectively, compared with the replete control. Meanwhile, higher lipid content was observed under anaerobic conditions combined with Mn-, N-, P- and S-deprived media (day 1) with 20%, 13%, 8% and 7% increases respectively compared with the control. This investigation, for the first time clearly, highlights the potential of P. kessleri as a sustainable biorefinery platform, for H 2 and fatty acid bio-production under anaerobic conditions. Key points • Parachlorella kessleri could provide a future sustainable biorefinery platform. • Nutrient-deprived anaerobic conditions blocked cell growth but differentially induced H 2 production. • Nutrient status, under both aerobic/anaerobic conditions, alters lipids and fatty acids profile of P. kessleri. • Nutrient-deprived (N- and Mn-) anaerobic conditions: future biorefinery platform.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-020-10930-3