Decolorization of Textile Effluent by Trametes hirsuta Bm-2 and lac-T as Possible Main Laccase-Contributing Gene

The decolorization of dye and textile effluent by Trametes hirsuta was studied in both induced and non-induced media. A removal of 70–100% of the color was achieved through adsorption and the action of laccases. Laccase activity was increased significantly with the addition of grapefruit peel (4000...

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Bibliographic Details
Published in:Current microbiology 2020-12, Vol.77 (12), p.3953-3961
Main Authors: Tapia-Tussell, Raul, Pereira‑Patrón, Alejandrina, Alzate-Gaviria, Liliana, Lizama-Uc, Gabriel, Pérez-Brito, Daisy, Solis‑Pereira, Sara
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biotechnology
Coloring Agents
Decoloring
Decolorization
Dyes
Effluents
Gene expression
Grapefruit
Isoenzymes
Laccase
Laccase - genetics
Life Sciences
Microbiology
Organic wastes
Polyporaceae
Textile industry wastes
Textile industry wastewaters
Textiles
Trametes - genetics
Trametes hirsuta
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Summary:The decolorization of dye and textile effluent by Trametes hirsuta was studied in both induced and non-induced media. A removal of 70–100% of the color was achieved through adsorption and the action of laccases. Laccase activity was increased significantly with the addition of grapefruit peel (4000 U/mL) and effluent with grapefruit peel (16,000 U/mL) in comparison with the basal medium (50 U/mL). Analysis of the expression of laccase isoenzymes lac-B and lac-T revealed clear differences in the expression of these genes. The low levels of expression of lac-B in all media suggest a basal or constitutive gene expression, whereas lac-T was over-expressed in the media with effluent, and showed an up/down regulation depending on culture conditions and time. The results obtained suggest that the lac-T gene of T. hirsuta is involved in the decolorization of dyes.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-020-02188-9