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Potential GnRH and steroidogenesis pathways in the scallop Patinopecten yessoensis
•Gonadotropin receptor like and steroidogenesis-related genes were found in scallops.•A potential GnRH signaling pathway in CPG may participate in regulating sex hormones.•CYP17A, HSD17B12 and HSD3B1 are likely involved in steroidogenesis in the scallop.•Correlation of E2/T with FOXL2 level indicate...
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| Published in: | The Journal of steroid biochemistry and molecular biology 2020-11, Vol.204, p.105756-105756, Article 105756 |
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| container_title | The Journal of steroid biochemistry and molecular biology |
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| creator | Zhang, Meiwei Wei, Huilan Liu, Tian Li, Wanru Li, Yajuan Wang, Shi Xing, Qiang Hu, Xiaoli Zhang, Lingling Bao, Zhenmin |
| description | •Gonadotropin receptor like and steroidogenesis-related genes were found in scallops.•A potential GnRH signaling pathway in CPG may participate in regulating sex hormones.•CYP17A, HSD17B12 and HSD3B1 are likely involved in steroidogenesis in the scallop.•Correlation of E2/T with FOXL2 level indicates presence of aromatase in scallops.
Gonadotropin-releasing hormone (GnRH) controls synthesis of sex steroid hormones through hypothalamic-pituitary-gonadal (HPG) axis in vertebrates. But in mollusks, research on GnRH and steroidogenesis pathways is still limited. In this study, we first identified two gonadotropin receptor like genes (LGR and LGR5L) and four steroidogenesis-related genes (CYP17A, HSD17B12, HSD3B1 and HSD3B2) in the scallop Patinopecten yessoensis. By examining the expression of 11 genes in the ganglia and/or gonad as well as the concentration of progesterone, testosterone and estradiol in the gonad, we postulate that a potential GnRH signaling pathway (GnRH-GnRHR-GPB5-LGR/LGR5L) in the cerebral and pedal ganglia (CPG) and steroidogenesis pathway (CYP17A, HSD17B12 and HSD3B1) in the gonad are involved in regulating sex steroid hormones. E2/T index that indicates aromatase activity is higher in the ovary than testis and is positively correlated with the expression of FOXL2 in the gonad, implying the presence of aromatase in the scallop. In addition, we confirmed that expression of most of the downstream genes in the two pathways was significantly elevated after injection of mature py-GnRH peptide. This study would contribute to a new understanding of the molecular basis underlying reproduction regulation by GnRH in mollusks. |
| doi_str_mv | 10.1016/j.jsbmb.2020.105756 |
| format | article |
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Gonadotropin-releasing hormone (GnRH) controls synthesis of sex steroid hormones through hypothalamic-pituitary-gonadal (HPG) axis in vertebrates. But in mollusks, research on GnRH and steroidogenesis pathways is still limited. In this study, we first identified two gonadotropin receptor like genes (LGR and LGR5L) and four steroidogenesis-related genes (CYP17A, HSD17B12, HSD3B1 and HSD3B2) in the scallop Patinopecten yessoensis. By examining the expression of 11 genes in the ganglia and/or gonad as well as the concentration of progesterone, testosterone and estradiol in the gonad, we postulate that a potential GnRH signaling pathway (GnRH-GnRHR-GPB5-LGR/LGR5L) in the cerebral and pedal ganglia (CPG) and steroidogenesis pathway (CYP17A, HSD17B12 and HSD3B1) in the gonad are involved in regulating sex steroid hormones. E2/T index that indicates aromatase activity is higher in the ovary than testis and is positively correlated with the expression of FOXL2 in the gonad, implying the presence of aromatase in the scallop. In addition, we confirmed that expression of most of the downstream genes in the two pathways was significantly elevated after injection of mature py-GnRH peptide. This study would contribute to a new understanding of the molecular basis underlying reproduction regulation by GnRH in mollusks.</description><identifier>ISSN: 0960-0760</identifier><identifier>EISSN: 1879-1220</identifier><identifier>DOI: 10.1016/j.jsbmb.2020.105756</identifier><identifier>PMID: 32979503</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>17β-Estradiol ; Animals ; Aromatase ; Female ; FOXL2 ; Ganglia ; GnRH signaling pathway ; Gonadal Steroid Hormones - metabolism ; Gonadotropin-releasing hormone ; Gonadotropin-Releasing Hormone - metabolism ; Gonadotropins ; Gonads - metabolism ; Hormones ; Hypothalamic-pituitary-gonadal axis ; Hypothalamus ; Male ; Mollusca ; Patinopecten yessoensis ; Pectinidae - metabolism ; Pedal ganglion ; Pituitary ; Pituitary (anterior) ; Progesterone ; sex steroid hormones ; Signal Transduction ; Steroid hormones ; Steroidogenesis ; Testosterone</subject><ispartof>The Journal of steroid biochemistry and molecular biology, 2020-11, Vol.204, p.105756-105756, Article 105756</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier BV Nov 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-50de9b2707e4f82633878b8921566919d165c9a2831ff4ef153fa8ae16d2cf673</citedby><cites>FETCH-LOGICAL-c387t-50de9b2707e4f82633878b8921566919d165c9a2831ff4ef153fa8ae16d2cf673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32979503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Meiwei</creatorcontrib><creatorcontrib>Wei, Huilan</creatorcontrib><creatorcontrib>Liu, Tian</creatorcontrib><creatorcontrib>Li, Wanru</creatorcontrib><creatorcontrib>Li, Yajuan</creatorcontrib><creatorcontrib>Wang, Shi</creatorcontrib><creatorcontrib>Xing, Qiang</creatorcontrib><creatorcontrib>Hu, Xiaoli</creatorcontrib><creatorcontrib>Zhang, Lingling</creatorcontrib><creatorcontrib>Bao, Zhenmin</creatorcontrib><title>Potential GnRH and steroidogenesis pathways in the scallop Patinopecten yessoensis</title><title>The Journal of steroid biochemistry and molecular biology</title><addtitle>J Steroid Biochem Mol Biol</addtitle><description>•Gonadotropin receptor like and steroidogenesis-related genes were found in scallops.•A potential GnRH signaling pathway in CPG may participate in regulating sex hormones.•CYP17A, HSD17B12 and HSD3B1 are likely involved in steroidogenesis in the scallop.•Correlation of E2/T with FOXL2 level indicates presence of aromatase in scallops.
Gonadotropin-releasing hormone (GnRH) controls synthesis of sex steroid hormones through hypothalamic-pituitary-gonadal (HPG) axis in vertebrates. But in mollusks, research on GnRH and steroidogenesis pathways is still limited. In this study, we first identified two gonadotropin receptor like genes (LGR and LGR5L) and four steroidogenesis-related genes (CYP17A, HSD17B12, HSD3B1 and HSD3B2) in the scallop Patinopecten yessoensis. By examining the expression of 11 genes in the ganglia and/or gonad as well as the concentration of progesterone, testosterone and estradiol in the gonad, we postulate that a potential GnRH signaling pathway (GnRH-GnRHR-GPB5-LGR/LGR5L) in the cerebral and pedal ganglia (CPG) and steroidogenesis pathway (CYP17A, HSD17B12 and HSD3B1) in the gonad are involved in regulating sex steroid hormones. E2/T index that indicates aromatase activity is higher in the ovary than testis and is positively correlated with the expression of FOXL2 in the gonad, implying the presence of aromatase in the scallop. In addition, we confirmed that expression of most of the downstream genes in the two pathways was significantly elevated after injection of mature py-GnRH peptide. This study would contribute to a new understanding of the molecular basis underlying reproduction regulation by GnRH in mollusks.</description><subject>17β-Estradiol</subject><subject>Animals</subject><subject>Aromatase</subject><subject>Female</subject><subject>FOXL2</subject><subject>Ganglia</subject><subject>GnRH signaling pathway</subject><subject>Gonadal Steroid Hormones - metabolism</subject><subject>Gonadotropin-releasing hormone</subject><subject>Gonadotropin-Releasing Hormone - metabolism</subject><subject>Gonadotropins</subject><subject>Gonads - metabolism</subject><subject>Hormones</subject><subject>Hypothalamic-pituitary-gonadal axis</subject><subject>Hypothalamus</subject><subject>Male</subject><subject>Mollusca</subject><subject>Patinopecten yessoensis</subject><subject>Pectinidae - metabolism</subject><subject>Pedal ganglion</subject><subject>Pituitary</subject><subject>Pituitary (anterior)</subject><subject>Progesterone</subject><subject>sex steroid hormones</subject><subject>Signal Transduction</subject><subject>Steroid hormones</subject><subject>Steroidogenesis</subject><subject>Testosterone</subject><issn>0960-0760</issn><issn>1879-1220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kE1rFEEQhhtRzLr6CwRp8OJl1v7Y6Y-DBwmaCIGEkJybnp4a08Ns99g1q-y_T68bPXjwVFA871vFQ8hbzjaccfVx3IzY7bqNYOK4aXWrnpEVN9o2XAj2nKyYVaxhWrEz8gpxZIxJyfVLciaF1bZlckVub_ICaYl-ohfp9pL61FNcoOTY5--QACPS2S8Pv_wBaUx0eQCKwU9TnumNX2LKM4TaQA-AmCFV_jV5MfgJ4c3TXJP7r1_uzi-bq-uLb-efr5ogjV6alvVgO6GZhu1ghJJ1azpjBW-Vstz2XLXBemEkH4YtDLyVgzceuOpFGJSWa_Lh1DuX_GMPuLhdxADT5BPkPTqx3SqlrZC2ou__Qce8L6l-VyltRGtkvb8m8kSFkhELDG4ucefLwXHmjsrd6H4rd0fl7qS8pt49de-7HfR_M38cV-DTCYAq42eE4jBESAH6WKo71-f43wOPgCKSJw</recordid><startdate>202011</startdate><enddate>202011</enddate><creator>Zhang, Meiwei</creator><creator>Wei, Huilan</creator><creator>Liu, Tian</creator><creator>Li, Wanru</creator><creator>Li, Yajuan</creator><creator>Wang, Shi</creator><creator>Xing, Qiang</creator><creator>Hu, Xiaoli</creator><creator>Zhang, Lingling</creator><creator>Bao, Zhenmin</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>202011</creationdate><title>Potential GnRH and steroidogenesis pathways in the scallop Patinopecten yessoensis</title><author>Zhang, Meiwei ; Wei, Huilan ; Liu, Tian ; Li, Wanru ; Li, Yajuan ; Wang, Shi ; Xing, Qiang ; Hu, Xiaoli ; Zhang, Lingling ; Bao, Zhenmin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-50de9b2707e4f82633878b8921566919d165c9a2831ff4ef153fa8ae16d2cf673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>17β-Estradiol</topic><topic>Animals</topic><topic>Aromatase</topic><topic>Female</topic><topic>FOXL2</topic><topic>Ganglia</topic><topic>GnRH signaling pathway</topic><topic>Gonadal Steroid Hormones - metabolism</topic><topic>Gonadotropin-releasing hormone</topic><topic>Gonadotropin-Releasing Hormone - metabolism</topic><topic>Gonadotropins</topic><topic>Gonads - metabolism</topic><topic>Hormones</topic><topic>Hypothalamic-pituitary-gonadal axis</topic><topic>Hypothalamus</topic><topic>Male</topic><topic>Mollusca</topic><topic>Patinopecten yessoensis</topic><topic>Pectinidae - metabolism</topic><topic>Pedal ganglion</topic><topic>Pituitary</topic><topic>Pituitary (anterior)</topic><topic>Progesterone</topic><topic>sex steroid hormones</topic><topic>Signal Transduction</topic><topic>Steroid hormones</topic><topic>Steroidogenesis</topic><topic>Testosterone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Meiwei</creatorcontrib><creatorcontrib>Wei, Huilan</creatorcontrib><creatorcontrib>Liu, Tian</creatorcontrib><creatorcontrib>Li, Wanru</creatorcontrib><creatorcontrib>Li, Yajuan</creatorcontrib><creatorcontrib>Wang, Shi</creatorcontrib><creatorcontrib>Xing, Qiang</creatorcontrib><creatorcontrib>Hu, Xiaoli</creatorcontrib><creatorcontrib>Zhang, Lingling</creatorcontrib><creatorcontrib>Bao, Zhenmin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Meiwei</au><au>Wei, Huilan</au><au>Liu, Tian</au><au>Li, Wanru</au><au>Li, Yajuan</au><au>Wang, Shi</au><au>Xing, Qiang</au><au>Hu, Xiaoli</au><au>Zhang, Lingling</au><au>Bao, Zhenmin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential GnRH and steroidogenesis pathways in the scallop Patinopecten yessoensis</atitle><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle><addtitle>J Steroid Biochem Mol Biol</addtitle><date>2020-11</date><risdate>2020</risdate><volume>204</volume><spage>105756</spage><epage>105756</epage><pages>105756-105756</pages><artnum>105756</artnum><issn>0960-0760</issn><eissn>1879-1220</eissn><abstract>•Gonadotropin receptor like and steroidogenesis-related genes were found in scallops.•A potential GnRH signaling pathway in CPG may participate in regulating sex hormones.•CYP17A, HSD17B12 and HSD3B1 are likely involved in steroidogenesis in the scallop.•Correlation of E2/T with FOXL2 level indicates presence of aromatase in scallops.
Gonadotropin-releasing hormone (GnRH) controls synthesis of sex steroid hormones through hypothalamic-pituitary-gonadal (HPG) axis in vertebrates. But in mollusks, research on GnRH and steroidogenesis pathways is still limited. In this study, we first identified two gonadotropin receptor like genes (LGR and LGR5L) and four steroidogenesis-related genes (CYP17A, HSD17B12, HSD3B1 and HSD3B2) in the scallop Patinopecten yessoensis. By examining the expression of 11 genes in the ganglia and/or gonad as well as the concentration of progesterone, testosterone and estradiol in the gonad, we postulate that a potential GnRH signaling pathway (GnRH-GnRHR-GPB5-LGR/LGR5L) in the cerebral and pedal ganglia (CPG) and steroidogenesis pathway (CYP17A, HSD17B12 and HSD3B1) in the gonad are involved in regulating sex steroid hormones. E2/T index that indicates aromatase activity is higher in the ovary than testis and is positively correlated with the expression of FOXL2 in the gonad, implying the presence of aromatase in the scallop. In addition, we confirmed that expression of most of the downstream genes in the two pathways was significantly elevated after injection of mature py-GnRH peptide. This study would contribute to a new understanding of the molecular basis underlying reproduction regulation by GnRH in mollusks.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32979503</pmid><doi>10.1016/j.jsbmb.2020.105756</doi><tpages>1</tpages></addata></record> |
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| subjects | 17β-Estradiol Animals Aromatase Female FOXL2 Ganglia GnRH signaling pathway Gonadal Steroid Hormones - metabolism Gonadotropin-releasing hormone Gonadotropin-Releasing Hormone - metabolism Gonadotropins Gonads - metabolism Hormones Hypothalamic-pituitary-gonadal axis Hypothalamus Male Mollusca Patinopecten yessoensis Pectinidae - metabolism Pedal ganglion Pituitary Pituitary (anterior) Progesterone sex steroid hormones Signal Transduction Steroid hormones Steroidogenesis Testosterone |
| title | Potential GnRH and steroidogenesis pathways in the scallop Patinopecten yessoensis |
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