Potential GnRH and steroidogenesis pathways in the scallop Patinopecten yessoensis

•Gonadotropin receptor like and steroidogenesis-related genes were found in scallops.•A potential GnRH signaling pathway in CPG may participate in regulating sex hormones.•CYP17A, HSD17B12 and HSD3B1 are likely involved in steroidogenesis in the scallop.•Correlation of E2/T with FOXL2 level indicate...

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Published in:The Journal of steroid biochemistry and molecular biology 2020-11, Vol.204, p.105756-105756, Article 105756
Main Authors: Zhang, Meiwei, Wei, Huilan, Liu, Tian, Li, Wanru, Li, Yajuan, Wang, Shi, Xing, Qiang, Hu, Xiaoli, Zhang, Lingling, Bao, Zhenmin
Format: Article
Language:English
Subjects:
17β-Estradiol
Animals
Aromatase
Female
FOXL2
Ganglia
GnRH signaling pathway
Gonadal Steroid Hormones - metabolism
Gonadotropin-releasing hormone
Gonadotropin-Releasing Hormone - metabolism
Gonadotropins
Gonads - metabolism
Hormones
Hypothalamic-pituitary-gonadal axis
Hypothalamus
Male
Mollusca
Patinopecten yessoensis
Pectinidae - metabolism
Pedal ganglion
Pituitary
Pituitary (anterior)
Progesterone
sex steroid hormones
Signal Transduction
Steroid hormones
Steroidogenesis
Testosterone
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Summary:•Gonadotropin receptor like and steroidogenesis-related genes were found in scallops.•A potential GnRH signaling pathway in CPG may participate in regulating sex hormones.•CYP17A, HSD17B12 and HSD3B1 are likely involved in steroidogenesis in the scallop.•Correlation of E2/T with FOXL2 level indicates presence of aromatase in scallops. Gonadotropin-releasing hormone (GnRH) controls synthesis of sex steroid hormones through hypothalamic-pituitary-gonadal (HPG) axis in vertebrates. But in mollusks, research on GnRH and steroidogenesis pathways is still limited. In this study, we first identified two gonadotropin receptor like genes (LGR and LGR5L) and four steroidogenesis-related genes (CYP17A, HSD17B12, HSD3B1 and HSD3B2) in the scallop Patinopecten yessoensis. By examining the expression of 11 genes in the ganglia and/or gonad as well as the concentration of progesterone, testosterone and estradiol in the gonad, we postulate that a potential GnRH signaling pathway (GnRH-GnRHR-GPB5-LGR/LGR5L) in the cerebral and pedal ganglia (CPG) and steroidogenesis pathway (CYP17A, HSD17B12 and HSD3B1) in the gonad are involved in regulating sex steroid hormones. E2/T index that indicates aromatase activity is higher in the ovary than testis and is positively correlated with the expression of FOXL2 in the gonad, implying the presence of aromatase in the scallop. In addition, we confirmed that expression of most of the downstream genes in the two pathways was significantly elevated after injection of mature py-GnRH peptide. This study would contribute to a new understanding of the molecular basis underlying reproduction regulation by GnRH in mollusks.
ISSN:0960-0760
1879-1220
DOI:10.1016/j.jsbmb.2020.105756