Bone marrow mesenchymal stem cells ameliorated kidney fibrosis by attenuating TLR4/NF-κB in diabetic rats

Diabetic nephropathy (DN) is a chronic inflammatory complication of diabetes mellitus, which becomes the most common cause of end-stage renal disease (ESRD). Recently, bone marrow mesenchymal stem cells (BMSCs) are considered as a promising therapy for DN. However, the protective mechanism of BMSCs...

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Bibliographic Details
Published in:Life sciences (1973) 2020-12, Vol.262, p.118385-8, Article 118385
Main Authors: Lin, Liya, Lin, Hefeng, Wang, Daijuanru, Bao, Zeying, Cai, Huabo, Zhang, Xiaoming
Format: Article
Language:English
Subjects:
Abnormalities
Animals
Blood
Blood Urea Nitrogen
BMSCs
Bone marrow
Bone marrow transplantation
Cells, Cultured
Collagen
Collagen (type I)
Creatinine
Creatinine - blood
Cytokines
Cytokines - metabolism
Diabetes
Diabetes mellitus
Diabetes Mellitus, Experimental - complications
Diabetes Mellitus, Experimental - therapy
Diabetic Nephropathies - prevention & control
Diabetic nephropathy
End-stage renal disease
Female
Fibrosis
Glucose - metabolism
Immunohistochemistry
In vivo methods and tests
Inflammation
Kidney diseases
Kidneys
Mesangial cells
Mesangial Cells - metabolism
Mesenchymal Stem Cell Transplantation - methods
Mesenchymal stem cells
Monocyte chemoattractant protein 1
Nephropathy
NF-kappa B - genetics
NF-κB protein
Rat
Rats
Rats, Sprague-Dawley
Renal function
Rodents
Staining
Stem cell transplantation
Stem cells
Streptozocin
TLR4 protein
TLR4/NF-κB
Toll-Like Receptor 4 - genetics
Toll-like receptors
Transplantation
Transplants & implants
Urea
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Summary:Diabetic nephropathy (DN) is a chronic inflammatory complication of diabetes mellitus, which becomes the most common cause of end-stage renal disease (ESRD). Recently, bone marrow mesenchymal stem cells (BMSCs) are considered as a promising therapy for DN. However, the protective mechanism of BMSCs on DN remains unclear. This study was done to explore the effect of a bone marrow stromal cell (BMSCs) transplant on DN rats and rat glomerular mesangial cells in high-glucose concentration. Diabetic rats were induced by a single intraperitoneal injection of streptozotocin (STZ) 65 mg/kg, then 4 × 106 BMSCs were transplanted in diabetic rats as the treatment group. Six weeks after BMSCs transplantation, blood serum creatinine (Scr) and blood urea nitrogen (BUN) were used to test renal function. Renal pathological examination was observed by HE staining, Masson staining, PAS staining and immunohistochemistry. The results demonstrated that BMSCs could dramatically improve renal function and collagen accumulation by reducing Scr, BUN, collagen I and IV expression and histopathological abnormalities in the diabetic kidneys. Furthermore, BMSCs could significantly attenuate the expression of TLR4/NF-κB and MCP-1 in vitro and in vivo (P 
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2020.118385