In situ functionalization of HPLC monolithic columns based on divinylbenzene-styrene-4-vinylbenzyl chloride

The properties of chromatographic columns are largely determined by functional groups located on the sorbent surface. For monolithic columns, surface functional groups can be created during synthesis stage or by chemical bonding with the complete surface of the sorbent. One of sorbent modification a...

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Bibliographic Details
Published in:Talanta (Oxford) 2020-12, Vol.220, p.121400-121400, Article 121400
Main Authors: Sotnikova, Yulia S., Patrushev, Yuri V., Sidelnikov, Vladimir N., Mazaeva, Anastasia A.
Format: Article
Language:English
Subjects:
Click reactions
HPLC
Mixed-mode chromatography
Monolithic columns
Porous polymer
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Summary:The properties of chromatographic columns are largely determined by functional groups located on the sorbent surface. For monolithic columns, surface functional groups can be created during synthesis stage or by chemical bonding with the complete surface of the sorbent. One of sorbent modification approach is to use on-column click reactions with surface reactive groups. In this study, the surface treatment of monolithic sorbent based on divinylbenzene (DVB), styrene (St) and 4-vinylbenzyl chloride (4VBC) copolymer by heterocyclic nitrogen-containing compounds 1-methylimidazole (1MI), 2-methylimidazole (2MI), 2-methylpyridine (2 MP) and 4-methylpyridine (4 MP) is described. The reaction of nitrogen-containing heterocycles with chloromethyl fragments on the surface results in formation of ion pairs and significantly changes the selectivity of monolithic columns. The chromatographic properties of prepared columns are studied. Modified columns can be operated in reversed-phase (RP) chromatography or in hydrophilic interaction liquid chromatography (HILIC) with different composition of the mobile phase. Separation examples of various chemical substances classes are given. [Display omitted] •Method for in situ functionalization of the organic monolith was developed.•The monolithic columns with pyridinium or imidazolium as functional group was prepared.•The functionalized columns enabled reversed-phase and hydrophilic interactions separation modes.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2020.121400