Effects of a biomimetic analog‐based experimental bonding system on caries‐affected and sound dentin

This study compared the ultrastructure, chemical composition, and proteases activity (PA) of sound (SD) and caries‐affected dentin (CAD) in the dentin hybrid layer after using an experimental bonding system containing pyromellitic dianhydride glycerol methacrylate and biomimetic analogs. The bonding...

Full description

Saved in:
Bibliographic Details
Published in:Microscopy research and technique 2020-12, Vol.83 (12), p.1610-1622
Main Authors: Gomes, Bianca Silva, Rossi, André Linhares, Silva, Eduardo Moreira, Moreira, Karla Tatiana Toro, Santos, Juliane Cucinello, Ferreira‐Pereira, Antônio, Portela, Maristela Barbosa
Format: Article
Language:English
Subjects:
Adhesion tests
adhesive
Adhesives
Biomimetics
Calcium
Calcium hydroxide
Calcium phosphates
Chemical composition
Demineralizing
Dental caries
Dentin
Dianhydrides
Electron diffraction
Evaluation
Glycerol
Hydroxyapatite
Interfaces
Nanoparticles
Polyacrylic acid
Proteolysis
remineralization
Slaked lime
Sound
Spectroscopy
Substrates
Transmission electron microscopy
Tricalcium phosphate
Ultrastructure
Variance analysis
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study compared the ultrastructure, chemical composition, and proteases activity (PA) of sound (SD) and caries‐affected dentin (CAD) in the dentin hybrid layer after using an experimental bonding system containing pyromellitic dianhydride glycerol methacrylate and biomimetic analogs. The bonding system used a three step and a total‐etch procedure. Polyacrylic acid (5%) and sodium trimetaphosphate (5%) were added to the primer and monocalcium phosphate monohydrate (9%), beta‐tricalcium phosphate (10.5%), and calcium hydroxide (0.5%) were added to the adhesive. Transmission electron microscopy (TEM) was used to evaluate the resultant structure, particularly the adhesive‐dentin and the demineralized‐SD interfaces. The chemical composition was evaluated through energy‐dispersive X‐ray spectroscopy (EDS) and selected area electron diffraction (SAED). The PA was measured with the Coomassie Blue‐G250 coloring test, and the PA data were analyzed by ANOVA. EDS identified the presence of isolated calcium phosphate nanoparticles in the demineralized region; however, the SAED analysis did not show any evidences of hydroxyapatite (HA) neoformation in SD and CAD. The biomimetic analog‐based adhesive system inhibited the activities of dentin proteases immediately after treatment. Additionally, the proteolytic activity on the affected dentin resembled that of the SD. In conclusion, no HA formed in the demineralized SD and CAD although there were calcium and phosphate deposits. The experimental adhesive system inhibited dentin proteases. The present study uses a new approach to investigate the hybrid layer behavior in dentin. The experimental adhesive system was synthesized and used on sound and affected‐caries dentin as the substrate to reproduce real clinical conditions. This study evaluated the biomimetic remineralization capacity of collagen fibrils and the proteases activity in the dentin hybrid layer in both sound (SD) and caries‐affected dentin (CAD) using a novel experimental bonding system containing pyromellitic dianhydride glycerol dimethacrylate (PMGDM) and biomimetic analogs. Representative TEM figure demonstrate the analysis after 3 months of the CAD with bioactive adhesives. The hydroxyapatite crystals were not detected by SAED. However, we suggest that there were mineral deposits close to the adhesive layer in the caries affected dentin.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.23557