Role of major histocompatibility complex II antigen‐presentation pathway genes in orange‐spotted grouper infected with Cryptocaryon irritans

Cryptocaryon irritans, a pathogen model for fish mucosal immunity, causes skin mucosal and systematic humoral immune response. Where and how MHC II antigen presentation occurs in fish infected with C. irritans remain unknown. In this study, the full‐length cDNA of the grouper cysteine protease CTSS...

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Bibliographic Details
Published in:Journal of fish diseases 2020-12, Vol.43 (12), p.1541-1552
Main Authors: Deng, Jun‐Jin, Xu, Shun, Li, Yan‐Wei, Xu, Dong‐Dong, Mo, Ze‐Quan, Li, Jia‐Zhou, Dan, Xue‐Ming, Luo, Xiao‐Chun
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigen presentation
Antigens
Antigens, Protozoan
Bass
Ciliophora Infections - immunology
Complementary DNA
Cryptocaryon irritans
Cysteine proteinase
Defence mechanisms
DNA
Epinephelus coioides
Fish
Fish Diseases - genetics
Fish Diseases - immunology
Fish Diseases - parasitology
Fish Proteins - genetics
Fish skin
Gene expression
Gene Expression Regulation - immunology
Genes
Hymenostomatida - physiology
Immune response
Immune response (humoral)
Immune system
Immunity
Immunity, Humoral
Immunity, Mucosal - genetics
Kidneys
Major histocompatibility complex
Major Histocompatibility Complex - genetics
Marine fishes
MHC II antigen presentation
Mucosal immunity
Pathogens
Serum
Spleen
Tissue
Tissues
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Summary:Cryptocaryon irritans, a pathogen model for fish mucosal immunity, causes skin mucosal and systematic humoral immune response. Where and how MHC II antigen presentation occurs in fish infected with C. irritans remain unknown. In this study, the full‐length cDNA of the grouper cysteine protease CTSS was cloned. The expression distributions of six genes (CTSB, CTSL, CTSS, GILT, MHC IIA and MHC IIB) involved in MHC II antigen presentation pathway were tested. These genes were highly expressed in systematic immune tissues and skin and gill mucosal‐associated immune tissues. All six genes were upregulated in skin at most time points. Five genes expected CTSS was upregulated in spleen at most time points. CTSB, CTSL and MHC IIA were upregulated in the gill and head kidney at some time points. These results indicate that the presentation of MHC II antigen intensively occurred in local infected skin and gill. Spleen, not head kidney, had the most extensive systematic antigen presentation. In skin, six genes most likely peaked at day 2, earlier than in spleen (5–7 days), marking an earlier skin antibody peak than any recorded in serum previously. This significant and earlier mucosal antigen presentation indicates that specific immune response occurs in local mucosal tissues.
ISSN:0140-7775
1365-2761
DOI:10.1111/jfd.13256