Danggui Niantong decoction induces apoptosis by activating Fas/caspase-8 pathway in rheumatoid arthritis fibroblast-like synoviocytes

OBJECTIVETo explore the effect of Danggui Niantong decoction (DGNTD) on cell apoptosis and TNF receptor super family 6 (Fas)/caspase-8 pathway in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS). METHODSFLS isolated from the synovial tissue of RA patients were cultured and identified usi...

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Published in:Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2020-08, Vol.40 (8), p.1119-1126
Main Authors: Zhao, Fan, Li, Jiayu, Lu, Qijin, Chen, Ensheng, Yuan, Lixia
Format: Article
Language:Chinese
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Summary:OBJECTIVETo explore the effect of Danggui Niantong decoction (DGNTD) on cell apoptosis and TNF receptor super family 6 (Fas)/caspase-8 pathway in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS). METHODSFLS isolated from the synovial tissue of RA patients were cultured and identified using immunofluorescence staining. The cells were treated with 10% blank serum (blank control group), 10% sera containing low, moderate or high doses of DGNTD, or 20 μmol/mL KR-33493 (a Fas inhibitor) combined with 10% serum containing high-dose DGNTD. MTT assay was used to detect the proliferation of the cells after the treatments. Apoptosis of the cells was detected at 48 h in each group using Hoechst 33342 staining and flow cytometry with annexin V-FITC/PI staining. The mRNA and protein expressions of Fas, FADD, caspase-8 and caspase-3 in the cells at 48 h were detected using qPCR and Western blotting. RESULTSImmunofluorescence staining identified the cultured cells as FLS. Treatment with DGNTD-containing sera sign
ISSN:1673-4254
DOI:10.12122/j.issn.1673-4254.2020.08.08