Optimal hyperosmotic agents for tissue immersion optical clearing in terahertz biophotonics

In this work, a thorough analysis of hyperosmotic agents for the immersion optical clearing (IOC) in terahertz (THz) range was performed. It was aimed at the selection of agents for the efficient enhancement of penetration depth of THz waves into biological tissues. Pulsed spectroscopy in the freque...

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Bibliographic Details
Published in:Journal of biophotonics 2020-12, Vol.13 (12), p.e202000297-n/a
Main Authors: Musina, Guzel R., Dolganova, Irina N., Chernomyrdin, Nikita V., Gavdush, Arsenii A., Ulitko, Vladislav E., Cherkasova, Olga P., Tuchina, Daria K., Nikitin, Pavel V., Alekseeva, Anna I., Bal, Natalia V., Komandin, Gennady A., Kurlov, Vladimir N., Tuchin, Valery V., Zaytsev, Kirill I.
Format: Article
Language:English
Subjects:
Absorptivity
brain tissue
Clearing
collimated transmission spectroscopy
Collimation
Diffusion
Diffusion rate
Frequency ranges
Glycerol
Immersion
immersion optical clearing
Nomograms
Optical properties
Penetration depth
Spectroscopy
Spectrum analysis
Submerging
Terahertz frequencies
terahertz penetration depth in tissue
terahertz spectroscopy
Tissues
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Summary:In this work, a thorough analysis of hyperosmotic agents for the immersion optical clearing (IOC) in terahertz (THz) range was performed. It was aimed at the selection of agents for the efficient enhancement of penetration depth of THz waves into biological tissues. Pulsed spectroscopy in the frequency range of 0.1 to 2.5 THz was applied for investigation of the optical properties of common IOC agents. Using the collimated transmission spectroscopy in visible range, binary diffusion coefficients of tissue water and agent in ex vivo rat brain tissue were measured. IOC agents were objectively compared using two‐dimensional nomogram, accounting for their THz‐wave absorption coefficients and binary diffusion coefficients. The results of this study demonstrate an interplay between the penetration depth enhancement and the diffusion rate and allow for pointing out glycerol as an optimal agent among the considered ones for particular applications in THz biophotonics. Nomogram of immersion optical clearing hyperosmotic agents, comparing their absorption at 1 THz and binary diffusion coefficient in rat brain tissue sample ex vivo.
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.202000297