Identification of Phosphatidylcholine Isomers in Imaging Mass Spectrometry Using Gas-Phase Charge Inversion Ion/Ion Reactions

Gas-phase ion/ion reactions have been enabled on a commercial dual source, hybrid QhFT-ICR mass spectrometer for use during imaging mass spectrometry experiments. These reactions allow for the transformation of the ion type most readily generated from the tissue surface to an ion type that gives imp...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-10, Vol.92 (19), p.13192-13201
Main Authors: Specker, Jonathan T, Van Orden, Steven L, Ridgeway, Mark E, Prentice, Boone M
Format: Article
Language:English
Subjects:
Animals
Anions
Brain
Cations
Chemistry
Gases - chemistry
Image processing
Inversion
Ion charge
Ionization
Ions
Ions - chemistry
Isomers
Lecithin
Lipid metabolism
Lipids
Mass Spectrometry
Mass spectroscopy
Metabolites
Molecular Structure
Neuroimaging
Phosphatidylcholine
Phosphatidylcholines - analysis
Rats
Reagents
Scientific imaging
Spectral analysis
Spectroscopy
Spectrum analysis
Stereoisomerism
Tissues
Workflow
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Summary:Gas-phase ion/ion reactions have been enabled on a commercial dual source, hybrid QhFT-ICR mass spectrometer for use during imaging mass spectrometry experiments. These reactions allow for the transformation of the ion type most readily generated from the tissue surface to an ion type that gives improved chemical structural information upon tandem mass spectrometry (MS/MS) without manipulating the tissue sample. This process is demonstrated via the charge inversion reaction of phosphatidylcholine (PC) lipid cations generated from rat brain tissue via matrix-assisted laser desorption/ionization (MALDI) with 1,4-phenylenedipropionic acid (PDPA) reagent dianions generated via electrospray ionization (ESI). Collision-induced dissociation (CID) of the resulting demethylated PC product anions allows for the determination of the lipid fatty acyl tail identities and positions, which is not possible via CID of the precursor lipid cations. The abundance of lipid isomers revealed by this workflow is found to vary significantly in different regions of the brain. As each isoform may have a unique cellular function, these results underscore the importance of accurately separating and identifying the many isobaric and isomeric lipids and metabolites that can complicate image interpretation and spectral analysis.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c02350