Effects in broiler hens of genetic lines differing in fertility, biotin supplementation, and age on relative abundance of oviductal transforming growth factor-β and carbonic anhydrase mRNA transcripts

•The effect of supplemental biotin on the oviductal expression of transforming growth factor-β (TGF-β) and carbonic anhydrase (CA) in young and old hens differing in fertility rate was studied.•The expression of TGF-β and CA mRNA in young hens was two-fold of that of the old birds.•The expression of...

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Published in:Animal reproduction science 2020-08, Vol.219, p.106480-106480, Article 106480
Main Authors: Rostami, S., Akhlaghi, A., Jafari Ahangari, Y., Ansari Pirsaraei, Z., Daryabari, H., Zahedi, M.R., Bagheri, M., Rafiee, P., Zhandi, M., Eghbalian, A.N., Saemi, F., Peebles, E.D.
Format: Article
Language:English
Subjects:
Hatchability
Immunity
Reproduction
Sperm storage tubule
Uterovaginal junction
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Summary:•The effect of supplemental biotin on the oviductal expression of transforming growth factor-β (TGF-β) and carbonic anhydrase (CA) in young and old hens differing in fertility rate was studied.•The expression of TGF-β and CA mRNA in young hens was two-fold of that of the old birds.•The expression of TGF-β and CA was respectively higher in low- and high-fertility line hens.•No correlation between fertility or hatchability rates and TGF-β or CA gene expression was found. There was evaluation of effects of biotin administration on oviductal abundance of transforming growth factor-β (TGF-β) and carbonic anhydrase (CA) mRNA transcript in younger and older broiler hens of relatively lesser and greater fertility lines. Additionally, effects of biotin supplementation on attenuation of age-related subfertility were evaluated. Hens from the relatively greater (Line D, n = 60) and lesser (Line B, n = 60) fertility rate line were randomly assigned to three treatment groups. Biotin was not or was administered in drinking water from 30 to 33 (younger age) and 53 to 56 (older age) wk of age to have access to no biotin (T0), or 0.3 (T1), or 0.45 (T2) mg/L of biotin. There was assessment the relative oviductal abundances of TGF-β and CA mRNA transcript abundances. Supplemental biotin and age had no effect on the relative abundance of oviductal TGF-β mRNA transcript in hens of Line D. There, however, was a ten-fold greater abundance of TGF-β in hens of the T0 group of Line B compared with Line D. Relative abundance of TGF-β mRNA transcript was greater in younger hens of Line B; however, biotin supplementation of older hens of the T2 group of Line B resulted in a similar TGF-β abundance to that of younger hens. Inconstant with the TGF-β abundance, CA abundance in hens of Line B was not affected by supplemental biotin or bird age. Overall, differences in TGF-β or CA abundances did not affect fertility of broiler hens.
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2020.106480