Yeast chromosomal engineering to improve industrially-relevant phenotypes

[Display omitted] •Comprehensive review of advances of the SCRaMbLE system.•The SRCaMbLE system has been successfully applied to accelerate phenotypic evolution.•The SRCaMbLE system provide a new research paradigm.•The SRCaMbLE system will be applied at the whole genome level in the future. Genome s...

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Bibliographic Details
Published in:Current opinion in biotechnology 2020-12, Vol.66, p.165-170
Main Authors: Jin, Jin, Jia, Bin, Yuan, Ying-Jin
Format: Article
Language:English
Subjects:
Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR-Cas Systems - genetics
Gene Editing
Genome, Fungal - genetics
Phenotype
Saccharomyces cerevisiae - genetics
Synthetic Biology
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Summary:[Display omitted] •Comprehensive review of advances of the SCRaMbLE system.•The SRCaMbLE system has been successfully applied to accelerate phenotypic evolution.•The SRCaMbLE system provide a new research paradigm.•The SRCaMbLE system will be applied at the whole genome level in the future. Genome structural variations enable microbes to evolve quickly under environmental stress. Recent efforts in synthetic biology have shown the ability of yeast chromosomal engineering to generate a larger scale of genome structural variations, which require a high efficiency of DNA rearrangement technology. In this review, we summarize the recent development of the SCRaMbLE system, an evolutionary approach, and the CRISPR/Cas9 technology to generate yeast genome rearrangement. Both technologies exhibit the powerful applications of chromosomal engineering to accelerate phenotypic evolution. We highlight several studies where yeast genome rearrangement has successfully improved industrially-relevant phenotypes, including the production of novel medicine, nutrition supplements, anti-tumor molecules, and the tolerance of environmental stress and drug resistance.
ISSN:0958-1669
1879-0429
DOI:10.1016/j.copbio.2020.07.003