Development of luminol-fluorescamine-PVP chemiluminescence system and its application to sensitive tyrosinase determination

Fluorescamine is a popular fluorescent probe. We report for the first time that luminol chemiluminescence (CL) can be enhanced by fluorescamine in the presence of PVP. The CL intensity of luminol-fluorescamine-PVP is about 26 times stronger than that of luminol. Both the removal of oxygen and the ad...

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Bibliographic Details
Published in:Talanta (Oxford) 2020-10, Vol.218, p.121177-121177, Article 121177
Main Authors: Wang, Chao, Chen, Yequan, Snizhko, Dmytro, Du, Fangxin, Ma, Xiangui, Lou, Baohua, Li, Jianping, Xu, Guobao
Format: Article
Language:English
Subjects:
Fluorescamine
Luminol chemiluminescence
Phenol
Polyvinylpyrrolidone
Tyrosinase activity
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Summary:Fluorescamine is a popular fluorescent probe. We report for the first time that luminol chemiluminescence (CL) can be enhanced by fluorescamine in the presence of PVP. The CL intensity of luminol-fluorescamine-PVP is about 26 times stronger than that of luminol. Both the removal of oxygen and the addition of superoxide dismutase (SOD) decrease CL intensity, thiourea and NaN3 have little effect on CL intensities, indicating that O2•− is critical for CL. Interestingly, o-quinone generated from phenol by tyrosinase obviously inhibited the CL intensity. Inspired by such quenching effect on the luminol-fluorescamine-PVP CL system, a sensitive CL sensing for the determination of tyrosinase activity was developed. The method can detect tyrosinase in the range of 0.07–1.5 μg mL−1 (0.19–4.02 U mL−1) with the detection limit of 0.035 μg mL−1 (0.094 U mL−1). Moreover, this method exhibits satisfied recoveries for the spiked human serum samples. [Display omitted] •Popular fluorescent probe fluorescamine has been first used for chemiluminescence.•Fluorescamine enhances luminol chemiluminescence dramatically.•PVP can raise luminol-fluorescamine chemiluminescence.•Catechol generated from phenol by tyrosinase remarkably quenches chemiluminescence.•Sensitive chemiluminescence tyrosinase detection has been realized.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2020.121177