Three‐dimensional analysis of injury conditions of single muscle fibers in small animals using phase‐contrast X‐ray imaging

Muscle damage can reduces the biological functions and lead to ultimately a disease state. For the reason, it is important to accurately check the state of an injury such as atrophy, and it is required to identify the state of fibers constituting the muscle. This study describes a novel method of an...

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Bibliographic Details
Published in:Microscopy research and technique 2021-01, Vol.84 (1), p.38-41
Main Authors: Lee, Jiwon, Jang, Sang‐Hun, Lee, Onseok
Format: Article
Language:English
Subjects:
Atrophy
Dimensional analysis
Drug development
Fibers
Injuries
Injury analysis
Mathematical analysis
mouse
Muscles
phase‐contrast X‐ray
single muscle fiber
synchrotron
volume rendering
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Summary:Muscle damage can reduces the biological functions and lead to ultimately a disease state. For the reason, it is important to accurately check the state of an injury such as atrophy, and it is required to identify the state of fibers constituting the muscle. This study describes a novel method of analyzing single muscle fibers with injury conditions in three‐dimensions. The muscle fibers of the mice were visualized using phase‐contrast X‐ray projection the microstructure. In additions, it was possible to confirm the status by quantitatively analyzing the injury severity of muscle fibers. Significantly, the muscle conditions of multiple individuals were individually determined. This study could contributes to areas where it is very important to identify microdetailed and quantitative changes of state, such as new drug development. We have suggested a new method to identify and visualize the injury to single muscle fibers by using a synchrotron radiation beam. This study was an approach to confirm the conditions of single muscle fibers, which were observed via X‐ray imaging.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.23561