Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions

Aims Several acidophilic bacteria have not been cultured, primarily owing to the lack of suitable culture methods under strong acidic conditions. This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong aci...

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Bibliographic Details
Published in:Journal of applied microbiology 2021-01, Vol.130 (1), p.157-164
Main Authors: Takano, C., Aoyagi, H.
Format: Article
Language:English
Subjects:
Acidophilic bacteria
Agar
Agar - chemistry
agar hydrolysis
agar plate
Bacteria
Bacteria - growth & development
Bacteria - isolation & purification
bacterial culture
Breaking
Colony Count, Microbial - methods
colony swarming
Culture Media - chemistry
E coli
Gellan gum
Hydrogen-Ion Concentration
isolation culture
pH effects
plate culture method
Plates
Polysaccharides, Bacterial - chemistry
Stress
Stress, Mechanical
uncultured bacteria
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Summary:Aims Several acidophilic bacteria have not been cultured, primarily owing to the lack of suitable culture methods under strong acidic conditions. This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions. Methods and Results To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined. The lower limit of available strength of AP and GP was 19·6 and 14·8 kPa, respectively. Medium composition slightly affected AP breaking stress, although GP with a high cationic concentration medium could not be prepared. Conclusions Assessment of the strength limits of AP and GP revealed that AP is not suitable for prolonged bacterial culture (≥72 h). Furthermore, GP was completely ineffective for bacterial culture under highly acidic conditions (≤pH 1·0). Significance and Impact of the Study Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.14768