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Scale-up of two-step acid-catalysed glycerol pretreatment for production of oleaginous yeast biomass from sugarcane bagasse by Rhodosporidium toruloides
Two-step acid-catalysed glycerol pretreatment improved sugar production while fed-batch cultivation enhanced microbial oil production on bagasse glucose and residual glycerol. [Display omitted] •Two-step acid-catalysed glycerol pretreatment was scaled-up to pilot scale.•Two-step glycerol pretreatmen...
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Published in: | Bioresource technology 2020-10, Vol.313, p.123666-123666, Article 123666 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Two-step acid-catalysed glycerol pretreatment improved sugar production while fed-batch cultivation enhanced microbial oil production on bagasse glucose and residual glycerol.
[Display omitted]
•Two-step acid-catalysed glycerol pretreatment was scaled-up to pilot scale.•Two-step glycerol pretreatment reduced glycerol usage compared to one-step process.•Two-step pretreatment improved the sugar yield compared to one-step process.•R. toruloides RP15 co-consumed bagasse glucose and glycerol for oil production.•Fed-batch cultivation improved oil production compared to batch cultivation.
Two-step dilute acid and acid-catalysed glycerol pretreatment was developed to maximise sugar yield from sugarcane bagasse. At the laboratory scale, dilute acid pretreatment at 130 °C followed by acid-catalysed glycerol pretreatment at 170 °C led to a total sugar (C5 + C6) yield of 82%, 31% higher than that from one-step acid-catalysed glycerol pretreatment. At the pilot scale, the two-step dilute acid and acid-catalysed glycerol pretreatment led to a maximum sugar yield of 74%, 13% higher than that from one-step pretreatment with 52% reduction in glycerol usage. The enzymatic hydrolysate containing glucose and residual glycerol were used to produce microbial oils by a Rhodosporidium toruloides strain. A fed-batch cultivation strategy led to the production of 44.8 g/L cell mass, including 26.6 g/L oil, 8.6 g/L protein and 12.7 mg/L carotenoid. The cell mass and oil yields were 19% higher than those from batch cultivation as feedstock inhibition and catabolite repression were alleviated. |
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ISSN: | 0960-8524 1873-2976 |
DOI: | 10.1016/j.biortech.2020.123666 |