A new practical approach for the biological treatment of a mixture of cheese whey and white wastewaters using Kefir grains

Kefir grains are a microbial consortium of different genera of bacteria and yeasts. In this study, the performance of Tunisian Kefir grains during the biological treatment of a mixture of Gouda cheese whey and white wastewaters (GCW) in ratio 1:1 with very high organic matter concentration is invest...

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Bibliographic Details
Published in:Environmental science and pollution research international 2020-09, Vol.27 (26), p.33127-33139
Main Authors: Elleuch, Lobna, Salem-Berrabah, Olfa Ben, Cherni, Yasmin, Sghaier-Hammami, Besma, Kasmi, Mariam, Botta, Cristian, Ouerghi, Ikram, Franciosa, Irene, Cocolin, Luca, Trabelsi, Ismail, Chatti, Abdelwaheb
Format: Article
Language:English
Subjects:
Aquatic Pollution
Atmospheric Protection/Air Quality Control/Air Pollution
Bacteria
Biogas
Biological activity
Biological treatment
Cheese
Consortia
Dairy products
Earth and Environmental Science
Ecotoxicology
Effluents
Environment
Environmental Chemistry
Environmental conditions
Environmental Health
Environmental science
Fingerprinting
Germination
High resistance
Kefir
Laboratories
Microorganisms
Milk
Organic matter
Research Article
Response surface methodology
rRNA 16S
Seed germination
Seedlings
Sequence analysis
Waste Water Technology
Wastewater
Water Management
Water Pollution Control
Whey
Yeasts
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Summary:Kefir grains are a microbial consortium of different genera of bacteria and yeasts. In this study, the performance of Tunisian Kefir grains during the biological treatment of a mixture of Gouda cheese whey and white wastewaters (GCW) in ratio 1:1 with very high organic matter concentration is investigated. The biological process was evaluated and optimized through the response surface methodology. Under the optimum conditions, Kefir grains concentration of 1.02%, temperature at 36.68 °C, and incubation time of 5.14 days, the removal efficiencies of COD, PO 4 3− , and NO 3 − were 87, 37.48, and 39.5%, respectively. Interestingly, the reusability tests of the grains proved not only their high resistance to harsh environmental conditions but also their great potential for more practical applications. Particularly, different strains were isolated from the grains and identified as Kluyveromyces marxianus , Lactoccocus lactis , Lactobacillus kefiri , and Bacillus spp. using 16S rDNA sequence analysis and rep-PCR fingerprinting. At the biological level, the raw GCW (RGCW) has a negative impact on the Hordeum vulgare both on seed germination, and on the growth parameters of seedlings. Interestingly, after Kefir grains treatment, the treated GCW (TGCW) allow a seedlings growth and germination rate similar to those soaked in water.
ISSN:0944-1344
1614-7499
DOI:10.1007/s11356-020-09549-8