Bimetallic organic framework-based aptamer sensors: a new platform for fluorescence detection of chloramphenicol

A fluorescence method for the quantitative detection of chloramphenicol (CAP) has been developed using phosphate and fluorescent dye 6-carboxy-x-rhodamine (ROX) double-labeled aptamers of CAP and the bimetallic organic framework nanomaterial Cu/UiO-66. Cu/UiO-66 was prepared by coordinate bonding of...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2020-09, Vol.412 (22), p.5273-5281
Main Authors: Lu, Zijing, Jiang, Yansong, Wang, Peng, Xiong, Weiwei, Qi, Baoping, Zhang, Yingkun, Xiang, Dongshan, Zhai, Kun
Format: Article
Language:English
Subjects:
Analytical Chemistry
Aptamers
Bimetals
Biochemistry
Characterization and Evaluation of Materials
Chemical compounds
Chemistry
Chemistry and Materials Science
Chloramphenicol
Chloromycetin
Copper
Electron transfer
Fluorescence
Fluorescence spectroscopy
Fluorescent dyes
Fluorescent indicators
Fluorophores
Food Science
Laboratory Medicine
Metal bonding
Metal-organic frameworks
Monitoring/Environmental Analysis
Nanomaterials
Nucleic acids
Phosphates
Research Paper
Rhodamine
Selectivity
Sensors
Single-stranded DNA
Spectrometry
Zirconium
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Summary:A fluorescence method for the quantitative detection of chloramphenicol (CAP) has been developed using phosphate and fluorescent dye 6-carboxy-x-rhodamine (ROX) double-labeled aptamers of CAP and the bimetallic organic framework nanomaterial Cu/UiO-66. Cu/UiO-66 was prepared by coordinate bonding of metal organic framework (MOF) nanomaterial UiO-66 with copper ions. Cu/UiO-66 contains a large number of metal defect sites, which can be combined with phosphate-modified nucleic acid aptamers through strong coordination between phosphate and zirconium to form “fluorescence turn-on” sensors. In the absence of CAP, all single-stranded aptamers were adsorbed on the surface of Cu/UiO-66 through π-π stacking between single-stranded DNA and Cu/UiO-66, which brings the ROX fluorophores and Cu/UiO-66 into close proximity. The ROX fluorescence of aptamers was then quenched by Cu/UiO-66 through photoinduced electron transfer (PET). In the presence of CAP, however, CAP reacted with nucleic acid aptamers to form a special spatial structure, in which the ROX fluorophores were far away from the MOF surface via a change in the spatial structure of the aptamers, and the fluorescence of ROX was able to be recovered. The quantitative detection of CAP can be achieved by measuring the fluorescence signal of ROX using synchronous scanning fluorescence spectrometry. Under optimum conditions, the fluorescence intensities of ROX exhibit a good linear dependence on the concentration of CAP in the range of 0.2–10 nmol/L, with a detection limit of 0.09 nmol/L. The method has advantages of high sensitivity, good selectivity, and a low limit of detection. Graphical abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-020-02737-y