Biological investigations of essential oils extracted from three Juniperus species and evaluation of their antimicrobial, antioxidant and cytotoxic activities

Aims To conduct biological investigations and to evaluate the antimicrobial and antioxidant activities of the essential oils (EOs) extracted from Juniperus communis, J. scopulorum and J. horizontalis; to screen their mechanisms of action by conducting the cell membrane permeability assay (CMP); and...

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Bibliographic Details
Published in:Journal of applied microbiology 2020-11, Vol.129 (5), p.1261-1271
Main Authors: Elshafie, H.S., Caputo, L., De Martino, L., Gruľová, D., Zheljazkov, V.Z., De Feo, V., Camele, I.
Format: Article
Language:English
Subjects:
Antibacterial activity
Antifungal activity
antimicrobial activities
Antimicrobial agents
antioxidant activity
Antioxidants
Assaying
biological investigations
cell membrane permeability
Cell membranes
Cytotoxicity
Essential oils
Evaluation
Fungi
Fungicides
Juniperus species
Membrane permeability
Monilinia fructicola
Neuroblastoma
Neuroblastoma cells
Oils & fats
Phytopathogenic fungi
phytopathogens
Plant diseases
Pseudomonas fluorescens
SH‐SY5Y
Toxicity
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Summary:Aims To conduct biological investigations and to evaluate the antimicrobial and antioxidant activities of the essential oils (EOs) extracted from Juniperus communis, J. scopulorum and J. horizontalis; to screen their mechanisms of action by conducting the cell membrane permeability assay (CMP); and to determine the possible cytotoxicity of the three EOs against human neuroblastoma cells (SH‐SY5Y). Methods and Results The antifungal activity was tested against four phytopathogenic fungi (Monilinia fructicola, Aspergillus niger, Penicillium expansum and Botrytis cinerea). The antibacterial activity was evaluated against two Gram‐positive (G+ve) (Bacillus megaterium and Clavibacter michiganensis) and three Gram‐negative (G−ve) bacterial strains (Pseudomonas fluorescens, P. syringae pv. phaseolicola and Xanthomonas campestris). Results showed that the three tested EOs have antifungal activity against M. fructicola and P. expansum and effective antibacterial activity against P. syringae pv. phaseolicola and B. megaterium. Moreover, the three EOs were evaluated for their ability to inhibit the growth of SH‐SY5Y cells with MTT assay. J. communis EO was the more effective with an IC50 of 53·7 μg ml−1. The antioxidant capacity of the three EO did not differ as measured by the DPPH assay. Conclusions The three tested juniper EOs showed promising antimicrobial and antioxidant activity and cytotoxic effects against human neuroblastoma cell line. Significance and Impact of the Study The outfindings from this research showed promising antimicrobial effects of the three oils against the majority of the tested phytopathogens with a potential to utilize them as natural alternatives to synthetic drugs, the cause of global environmental problems, pathogen resistance and difficulty to control many post‐harvest plant diseases.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.14723