Immunization with cytolethal distending toxin B produces autoantibodies to vinculin and small bowel bacterial changes in a rat model of postinfectious irritable bowel syndrome

Background Recent data substantiate the importance of acute gastroenteritis in the development of irritable bowel syndrome (IBS). An animal model of postinfectious IBS determined the importance of cytolethal distending toxin B (CdtB) during live Campylobacter jejuni infection and its development of...

Full description

Saved in:
Bibliographic Details
Published in:Neurogastroenterology and motility 2020-10, Vol.32 (10), p.e13875-n/a
Main Authors: Morales, Walter, Triantafyllou, Konstantinos, Parodi, Gonzalo, Weitsman, Stacy, Park, Sung Chul, Rezaie, Ali, Pichetshote, Nipaporn, Lin, Eugenia, Pimentel, Mark
Format: Article
Language:English
Subjects:
Animal models
Antibodies
Autoantibodies
Autoimmunity
Cytolethal distending toxin
cytolethal distending toxin B
Gastroenteritis
Immunization
Injection
Irritable bowel syndrome
Localization
Phenotypes
postinfectious irritable bowel syndrome
rat model
Rodents
Small intestine
Vinculin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Recent data substantiate the importance of acute gastroenteritis in the development of irritable bowel syndrome (IBS). An animal model of postinfectious IBS determined the importance of cytolethal distending toxin B (CdtB) during live Campylobacter jejuni infection and its development of autoimmunity to vinculin. In this study, we examine whether subcutaneous exposure to CdtB alone is sufficient to produce the postinfectious IBS effect and autoimmunity. Methods Sixty adult Sprague Dawley rats were randomized into 2 groups to receive subcutaneous injection of either CdtB or vehicle and administered a booster injection of the same product 3 weeks later. Serum was collected for anti‐CdtB and anti‐vinculin titers. Duodenal and ileal luminal contents for total eubacterial qPCR, and ileal bowel segments were harvested for vinculin and ileal expression. In a second experiment, 4 adult, Sprague Dawley rats were injected with either Cy7‐labeled anti‐CdtB and anti‐vinculin antibodies were injected into the tail vein and imaged to determine organ localization of the antibodies. Key Results Rats that received CdtB increased in serum anti‐CdtB after injection. CdtB exposure also precipitated significant elevation in anti‐vinculin antibodies (P 
ISSN:1350-1925
1365-2982
DOI:10.1111/nmo.13875