Structural features of pectic polysaccharides from stems of two species of Radix Codonopsis and their antioxidant activities

In this study, two pectic polysaccharides from stems of Codonopsis pilosula (CPSP-1) and C. tangshen (CTSP-1) were obtained by ion exchange chromatography and gel filtration. The molecular weight of CPSP-1 and CTSP-1 were 13.1 and 23.0 kDa, respectively. The results of structure elucidation indicate...

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Bibliographic Details
Published in:International journal of biological macromolecules 2020-09, Vol.159, p.704-713
Main Authors: Zou, Yuan-Feng, Zhang, Yan-Yun, Paulsen, Berit Smestad, Rise, Frode, Chen, Zheng-Li, Jia, Ren-Yong, Li, Li-Xia, Song, Xu, Feng, Bin, Tang, Hua-Qiao, Huang, Chao, Yin, Zhong-Qiong
Format: Article
Language:English
Subjects:
Animals
Antioxidant activity
Antioxidants - chemistry
Antioxidants - isolation & purification
Antioxidants - pharmacology
Cell Line
Codonopsis - chemistry
Codonopsis stem polysaccharides
Magnetic Resonance Spectroscopy
Molecular Structure
Molecular Weight
Monosaccharides
Plant Stems - chemistry
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Structural characterization
Structure-Activity Relationship
Swine
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Summary:In this study, two pectic polysaccharides from stems of Codonopsis pilosula (CPSP-1) and C. tangshen (CTSP-1) were obtained by ion exchange chromatography and gel filtration. The molecular weight of CPSP-1 and CTSP-1 were 13.1 and 23.0 kDa, respectively. The results of structure elucidation indicated that both CPSP-1 and CTSP-1 are pectic polysaccharides with long homogalacturonan regions (HG) (some of galacturonic acid units were methyl esterified) and rhamnogalacturonan I (RG-I) regions. Side chains for CTSP-1 are both arabinogalactan type I (AG-I) and type II (AG-II), while CPSP-1 only has AG-II. The biological test demonstrated that CPSP-1 and CTSP-1 displayed an antioxidant property through mediating the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress induced oxidative damages and cell viability suppression. CPSP-1 and CTSP-I showed different bioactivities and mechanisms, which may be due to the difference in their structures. •Polysaccharides from stem of Codonopsis pilosula and C. tangshen were purified.•These two pectic polysaccharides have different structural features.•Both polysaccharides have strong antioxidant activity.•The difference of biological activities may be related to the structure.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2020.05.083