Determination of glycosylation degree for glycoconjugate vaccines using a solid‐phase extraction combined with liquid chromatography and tandem mass spectrometry method

In this study, a solid‐phase extraction with liquid chromatography and tandem mass spectrometry method was developed to determine the degree of glycosylation of glycosylation sites and the ratio of free carrier protein to total carrier protein for glycoconjugate vaccines. To remove and enrich the gl...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2020-07, Vol.43 (14), p.2880-2888
Main Authors: Long, Zhen, Li, Maoguang, Dahl, Jeffrey, Guo, Zhimou, Li, Yanan, Hao, Hongyuan, Li, Yueqi, Li, Changkun, Mao, Qiqi, Huang, Taohong
Format: Article
Language:English
Subjects:
Chromatography
CRM197
glycoconjugate vaccines, glycosylation degree
Ions
Liquid chromatography
Mass spectrometry
Peptides
Proteins
Quality assessment
Scientific imaging
Size exclusion chromatography
solid phase extraction
Spectroscopy
tetanus
Vaccines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, a solid‐phase extraction with liquid chromatography and tandem mass spectrometry method was developed to determine the degree of glycosylation of glycosylation sites and the ratio of free carrier protein to total carrier protein for glycoconjugate vaccines. To remove and enrich the glycosylated peptides, a solid‐phase extraction method was developed, optimized, and hyphenated to liquid chromatography−tandem mass spectrometry. The developed solid‐phase extraction with liquid chromatography−tandem mass spectrometry method was shown to possess a wide linear dynamic range (0.03−100 μg/mL), a high sensitivity (0.03 μg/mL for CRM197), good interday and intra‐day precision (relative standard deviation of peak area 
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202000075