Supplementing a skimmed milk–egg yolk‐based extender with L‐carnitine helps maintain the motility, membrane integrity and fertilizing capacity of chilled ram sperm

This study examines the effect of L‐carnitine (LC) on chilled ram semen stored for up to 96 hr. Semen samples were collected, placed in a skimmed milk + 6% egg yolk extender, pooled, aliquoted and diluted with the same extender supplemented with different LC concentration: 0 (control), 1 mM (LC1), 2...

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Bibliographic Details
Published in:Reproduction in domestic animals 2020-07, Vol.55 (7), p.805-813
Main Authors: Galarza, Diego A., López‐Sebastián, Antonio, Santiago‐Moreno, Julián
Format: Article
Language:English
Subjects:
Acrosome
Animals
Artificial insemination
Carnitine
Carnitine - pharmacology
Cell Membrane
chilled
Cooling
Female
fertility
Fertilization
Fluorescence
Insemination, Artificial - veterinary
L‐carnitine
Male
Membranes
Milk
Mitochondria
Motility
Pregnancy
ram sperm
Reproduction (biology)
Semen
Semen Analysis - veterinary
Semen Preservation - methods
Semen Preservation - veterinary
Sheep, Domestic
skimmed milk
Sperm
Sperm Motility - drug effects
Spermatozoa - drug effects
Velocity
Yolk
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Summary:This study examines the effect of L‐carnitine (LC) on chilled ram semen stored for up to 96 hr. Semen samples were collected, placed in a skimmed milk + 6% egg yolk extender, pooled, aliquoted and diluted with the same extender supplemented with different LC concentration: 0 (control), 1 mM (LC1), 2.5 mM (LC2.5), 5 mM (LC5), 7.5 mM (LC7.5) or10 mM (LC10). Sperm kinetics and membranes (plasma, acrosome and mitochondrial) were examined using the CASA system and triple fluorescence staining (PI/ PNA‐FITC/Mitotracker). The progressive motility was greater (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13687