Fluorescent Properties of Carboxyfluorescein Bifluorophores

Bright fluorescent probes with enhanced intensities in the fluorescein channel are of great value for plenty of biological applications. To design effective probes one should introduce as many as possible fluorophores to the biomolecule while leaving its native structure as intact as possible. To re...

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Bibliographic Details
Published in:Journal of fluorescence 2020-05, Vol.30 (3), p.629-635
Main Authors: Povedailo, Vladimir A., Lysenko, Ivan L., Tikhomirov, Sergei A., Yakovlev, Dmitrii L., Tsybulsky, Dmitry A., Kruhlik, Aliaksandr S., Fan, Fan, Martynenko-Makaev, Yury V., Sharko, Olga L., Duong, Pham V., Minh, Pham H., Shmanai, Vadim V.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biomedicine
Biomolecules
Biophysics
Biotechnology
Chemical compounds
Fluorescein
Fluorescent indicators
Isomers
Original Article
Rigid structures
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Summary:Bright fluorescent probes with enhanced intensities in the fluorescein channel are of great value for plenty of biological applications. To design effective probes one should introduce as many as possible fluorophores to the biomolecule while leaving its native structure as intact as possible. To reach this compromise, we designed and synthesized fluorescein bifluorophores on the 3,5-diaminobenzoic acid scaffold, which allows for insertion of two fluorophores at one modification site of a biomolecule. Rigid structure of the branching linker group allows to minimize self-quenching the fluorophores. However, despite the structure similarities of fluorescein isomers (5-FAM and 6-FAM), different photophysical behavior was observed for the corresponding bifluorophores. Here we made efforts to get insight into these effects with the focus on the media viscosity impact.
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-020-02535-w