Effect of curcumin on growth, biofilm formation and virulence factor gene expression of Porphyromonas gingivalis

[Abstract] Porphyromonas gingivalis is a keystone pathogen and major colonizer in host tissue which plays a pivotal role in periodontitis among the other polymicrobial infections. Increasing facts demonstrate that curcumin has antibacterial activity and anti-biofilm effect against the periodontopath...

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Bibliographic Details
Published in:Odontology 2021-01, Vol.109 (1), p.18-28
Main Authors: Kumbar, Vijay M., Peram, Malleswara Rao, Kugaji, Manohar S., Shah, Tejas, Patil, Sanjivani P., Muddapur, Uday M., Bhat, Kishore G.
Format: Article
Language:jpn ; eng
Subjects:
Adhesins, Bacterial - genetics
Antibacterial activity
Biofilms
Curcumin
Curcumin - pharmacology
Dentistry
Gene Expression
Medicine
Minimum inhibitory concentration
Oral and Maxillofacial Surgery
Original Article
Periodontal diseases
Periodontitis
Porphyromonas gingivalis
Virulence
Virulence factors
Virulence Factors - genetics
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Summary:[Abstract] Porphyromonas gingivalis is a keystone pathogen and major colonizer in host tissue which plays a pivotal role in periodontitis among the other polymicrobial infections. Increasing facts demonstrate that curcumin has antibacterial activity and anti-biofilm effect against the periodontopathogens through diverse mechanisms that have a positive impact on periodontal health. The present study was aimed to elucidate the effect of curcumin on biofilm formation and virulence factor gene expression of P. gingivalis. By using gene expression studies, we exploited the mechanism of anti-biofilm effects of curcumin on P. gingivalis. The minimum inhibitory concentration and minimum bactericidal concentration of curcumin for both ATCC and clinical strains of P. gingivalis were found to be 62.5 and 125 μg ml-1 respectively. Curcumin prevented bacterial adhesion and biofilm formation in a dose-dependent manner. Further, curcumin attenuated the virulence of P. gingivalis by reducing the expression of genes coding for major virulence factors, including adhesions (fimA, hagA, and hagB) and proteinases (rgpA, rgpB, and kgp). The results indicated that curcumin has shown anti-biofilm as well as antibacterial activity against P. gingivalis. Further, curcumin because of its pleiotropic actions could be a simple and inexpensive therapeutic strategy in the treatment of periodontal disease.
ISSN:1618-1247
1618-1255
DOI:10.1007/s10266-020-00514-y