Overexpression of microRNA-141 inhibits osteoporosis in the jawbones of ovariectomized rats by regulating the Wnt/β-catenin pathway

•miR-141 overexpression improved osteoporosis of jawbones in ovariectomized rats.•miR-141 overexpression decreased RANKL, BGP and TRAP levels in serum.•miR-141 overexpression increased Runx2 and OSX expression in jawbones.•miR-141 regulated Wnt/β-catenin pathway in jawbones of ovariectomized rats. T...

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Published in:Archives of oral biology 2020-05, Vol.113, p.104713-104713, Article 104713
Main Authors: Liu, Tong-jun, Guo, Jian-lian
Format: Article
Language:English
Subjects:
Dentistry
miR-141
Osteoporosis
Ovariectomized rats
Wnt/β-catenin pathway
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Summary:•miR-141 overexpression improved osteoporosis of jawbones in ovariectomized rats.•miR-141 overexpression decreased RANKL, BGP and TRAP levels in serum.•miR-141 overexpression increased Runx2 and OSX expression in jawbones.•miR-141 regulated Wnt/β-catenin pathway in jawbones of ovariectomized rats. This work was aimed to investigate the effect of microRNA-141 (miR-141) overexpression in the jawbones of ovariectomized-induced osteoporosis rats and investigate the role of miR-141 in the Wnt/β-catenin pathway. Twenty-four female rats were randomly divided into the sham group, ovariectomized osteoporosis group (OP), miR-141 agonist group (miR-141), and miR-141 scramble group (Scramble). Bone mineral density (BMD) and pathological changes of the jaw were detected. Serum receptor activator of nuclear factor-B ligand (RANKL), osteoprotegerin, tartrate-resistant acid phosphatase (TRAP), and bone gla protein (BGP) levels were tested by ELISA. The expression of Runt-related transcription factor 2 (Runx2), and Osterix measured by immunohistochemistry and the expression of Wnt, β-catenin, and Dickkopf1 (DKK1) proteins was measured by Western blot. Furhter, the Wnt agonist DKK2-C2, Wnt inhibitor Endostar were used to verify the effect of miR-141 overexpression on the Wnt/β-catenin pathway. Compared with the OP group, the content of osteoprotegerin increased while the levels of RANKL, BGP, TRAP decreased in the miR-141 and DKK2-C2 groups (p 
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2020.104713