Polydopamine-coated nucleic acid nanogel for siRNA-mediated low-temperature photothermal therapy

Photothermal therapy (PTT) normally requires to maintain the temperature of tumor lesions above 50 °C, which potentially induces local inflammation and tumor metastasis. To avoid these side effects, it is vital to achieve effective antitumor efficacy at relatively low temperature (42–45 °C) during t...

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Bibliographic Details
Published in:Biomaterials 2020-07, Vol.245, p.119976-119976, Article 119976
Main Authors: Ding, Fei, Gao, Xihui, Huang, Xiangang, Ge, Huan, Xie, Miao, Qian, Jiwen, Song, Jie, Li, Yuehua, Zhu, Xinyuan, Zhang, Chuan
Format: Article
Language:English
Subjects:
Gene silencing
Hyperthermia, Induced
Indoles
Low temperature
Nanogels
Nucleic Acids
Phototherapy
Photothermal Therapy
Polydopamine coating
Polyethylene Glycols
Polyethyleneimine
Polymers
RNA, Small Interfering
RNase resistance
siRNA delivery
Temperature
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Summary:Photothermal therapy (PTT) normally requires to maintain the temperature of tumor lesions above 50 °C, which potentially induces local inflammation and tumor metastasis. To avoid these side effects, it is vital to achieve effective antitumor efficacy at relatively low temperature (42–45 °C) during the PTT treatment. Herein, we design a polydopamine (PDA)-coated nucleic acid nanogel as a therapeutic complex for siRNA-mediated low-temperature PTT. First, siRNAs that target the heat-shock-protein 70 (Hsp70) serve as crosslinkers to guide the DNA-grafted polycaprolactone (DNA-g-PCL) assemble into nanosized hydrogel particles through nucleic acid hybridization. Thereafter, the obtained siRNA-embedded nanogels are further coated with a thin layer of polydopamine, which not only protects the nanogels against enzymatic degradation but also endows the nanogels with excellent photothermal conversion capacity under near infrared (NIR) light irradiation. After surface PEGylation, this triple shield siRNA delivery complex shows the capability of effective ablating the tumor under relatively mild condition. A polydopamine-coated nucleic acid nanogel bearing siRNAs is developed, which protects the siRNA from nuclease digestion, prolongs blood circulation time of nanogel, and significantly enhances its target gene silencing capability, making the polydopamine-coated nanogel as a promising delivery system for siRNA-mediated low-temperature photothermal therapy. [Display omitted]
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2020.119976