Expansion and improvement of MALDI-TOF MS databases for accurate identification of Achromobacter species

Different MALDI-TOF MS databases were evaluated for the identification of Achromobacter species. The in-house and extended database generated in this study rendered more accurate identification (58/64 and 57/64 isolates, respectively) in comparison with the Bruker commercial database (42/64 isolates...

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Bibliographic Details
Published in:Journal of microbiological methods 2020-05, Vol.172, p.105889-105889, Article 105889
Main Authors: Papalia, Mariana, Figueroa-Espinosa, Roque, Steffanowski, Carla, Barberis, Claudia, Almuzara, Marisa, Barrios, Rubén, Vay, Carlos, Gutkind, Gabriel, Di Conza, José, Radice, Marcela
Format: Article
Language:English
Subjects:
Achromobacter identification
Achromobacter species
MALDI-TOF MS
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Summary:Different MALDI-TOF MS databases were evaluated for the identification of Achromobacter species. The in-house and extended database generated in this study rendered more accurate identification (58/64 and 57/64 isolates, respectively) in comparison with the Bruker commercial database (42/64 isolates), especially in those infrequent species that are not available or poorly represented. •MALDI-TOF MS proved to be a reliable identification tool at the genus level in Achromobacter•Expansion of MALDI-TOF MS databases rendered more accurate identification at species level•Discriminatory peaks were selected in order to differentiate among highly related species
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2020.105889