Immobilization of horseradish peroxidase onto Purolite® A109 and its anthraquinone dye biodegradation and detoxification potential

Horseradish peroxidase (HRP) is a highly specific enzyme with great potential for use in the decolorization of synthetic dyes. A comprehensive study of HRP immobilization using various techniques such as adsorption and covalent immobilization on the novel carrier Purolite® A109 with a special focus...

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Bibliographic Details
Published in:Biotechnology progress 2020-07, Vol.36 (4), p.e2991-n/a
Main Authors: Šekuljica, Nataša Ž., Jovanović, Jelena R., Jakovetić Tanasković, Sonja M., Ognjanović, Nevena D., Gazikalović, Ivana V., Knežević‐Jugović, Zorica D., Mijin, Dušan Ž.
Format: Article
Language:English
Subjects:
Acute toxicity
Adsorption
Anthraquinone
Anthraquinone dyes
Artemia
Biodegradation
Decoloring
Decolorization
Detoxification
Dyes
Horseradish peroxidase
Immobilization
Industrial plants
Industrial wastes
Industrial wastewater
Peroxidase
Phytotoxicity
Seeds
Stability analysis
synthetic dyes
Toxicity
wastewater
Wastewater treatment
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Summary:Horseradish peroxidase (HRP) is a highly specific enzyme with great potential for use in the decolorization of synthetic dyes. A comprehensive study of HRP immobilization using various techniques such as adsorption and covalent immobilization on the novel carrier Purolite® A109 with a special focus on enzymatic decolorization and toxicity of artificially colored wastewater. The immobilized preparations with an activity of 156.21 ± 1.41 U g−1 and 85.71 ± 1.62 U g−1 after the HRP adsorption and covalent immobilization, respectively, were obtained. Stability and reusability of the immobilized preparations were also evaluated. A noteworthy decolorization level (~90%) with immobilized HRP was achieved. Phytotoxicity testing using Mung bean seeds and acute toxicity assay with Artemia salina has confirmed the applicability of the obtained immobilized preparation in industrial wastewater plants for the treatment of colored wastewater.
ISSN:8756-7938
1520-6033
DOI:10.1002/btpr.2991