Highly sensitive electrochemiluminescence biosensor for VEGF165 detection based on a g-C3N4/PDDA/CdSe nanocomposite

In this work, an electrochemiluminescence (ECL) biosensor was fabricated for the selective detection of vascular endothelial growth factor (VEGF 165 ). g-C 3 N 4 /PDDA/CdSe nanocomposites were used as the ECL substrate. Then, DNA labeled at the 5′ end with amino groups (DNA 1 ) was immobilized on th...

Full description

Saved in:
Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2020-05, Vol.412 (13), p.3073-3081
Main Authors: Cheng, Jin-long, Liu, Xing-Pei, Chen, Jing-Shuai, Mao, Chang-jie, Jin, Bao-Kang
Format: Article
Language:English
Subjects:
Amino groups
Analytical Chemistry
Biochemistry
Biosensors
Carbon nitride
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Deoxyribonucleic acid
DNA
Electrochemiluminescence
Electrodes
Energy transfer
Food Science
Glassy carbon
Growth factors
Laboratory Medicine
Monitoring/Environmental Analysis
Nanocomposites
Research Paper
Selectivity
Substrates
Vascular endothelial growth factor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this work, an electrochemiluminescence (ECL) biosensor was fabricated for the selective detection of vascular endothelial growth factor (VEGF 165 ). g-C 3 N 4 /PDDA/CdSe nanocomposites were used as the ECL substrate. Then, DNA labeled at the 5′ end with amino groups (DNA 1 ) was immobilized on the surface of g-C 3 N 4 /PDDA/CdSe nanocomposite-modified glassy carbon electrode (GCE) by amido linkage. AuNP-labeled target DNA (Au-DNA 2 ) could hybridize with DNA 1 to form a double strand. The ECL of the g-C 3 N 4 /PDDA/CdSe nanocomposite was efficiently quenched due to the resonance energy transfer between CdSe QDs and Au NPs. After VEGF 165 was recognized and bound by Au-DNA 2 , the double helix was disrupted, and the energy transfer was broken. In this case, Au-DNA 2 was released from the electrode surface, and the ECL intensity recovered to a higher level. Under optimal conditions, this ECL biosensor possesses excellent selectivity, accuracy, and stability for VEGF 165 detection in a linear range of 2 pg mL −1 to 2 ng mL −1 with a detection limit of 0.68 pg mL −1 . In addition, this assay has been successfully applied to the determination of VEGF 165 in serum samples. Graphical abstract Schematic representation of the electrochemiluminescence sensor based on a g-C3N4/PDDA/CdSe nanocomposite, which can be determined in the concentration of vascular endothelial growth factor in serum.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-020-02552-5