Significance of Luminex-based single antigen class II bead assay and its mean fluorescence intensity in renal transplant cases; a retrospective observation in 97 cases

The objective of this study was to determine the mean fluorescence intensity (MFI) values of class II Luminex single antigen bead (L-SAB) assay and compare these MFI values with cell-based complement-dependent cytotoxicity crossmatch with anti-human globulin (CDC-AHG-XM) and IgG-B-cell flow cytometr...

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Bibliographic Details
Published in:Journal of immunoassay & immunochemistry 2020-05, Vol.41 (3), p.322-336
Main Authors: Pande, Amit, Pandey, Prashant, Kumar Devra, Amit, Kumar Sinha, Vijay, Prasad Bhatt, Anil
Format: Article
Language:English
Subjects:
Adult
Antibodies
Antibodies - immunology
Antigens
Assaying
CDC-AHG-XM
Cytotoxicity
Diagnostic systems
donor-specific anti-HLA antibodies
DSA
Female
Flow cytometry
Fluorescence
Globulins
Histocompatibility antigen HLA
Histocompatibility Antigens Class II - analysis
Histocompatibility Antigens Class II - immunology
Histocompatibility Testing
HLA
Humans
Immunoglobulin G
Kidney Transplantation
Leukocytes
Lymphocytes B
Male
mean fluorescence intensity
Microscopy, Fluorescence
Middle Aged
Retrospective Studies
Sensitivity
Toxicity
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Summary:The objective of this study was to determine the mean fluorescence intensity (MFI) values of class II Luminex single antigen bead (L-SAB) assay and compare these MFI values with cell-based complement-dependent cytotoxicity crossmatch with anti-human globulin (CDC-AHG-XM) and IgG-B-cell flow cytometry crossmatch (FC-XM) results and explore the near-accurate MFI-cutoff values of positive cell-based crossmatch results. This retrospective study was an analysis in 97 renal transplant recipients, who were tested for the presence of DSA by CDC-AHG-XM and IgG-B-cell-FC-XM methods with their corresponding donor as well as for anti-human leukocyte antigen (HLA) antibody detection using a sensitive L-SAB assay. In the group having DSA MFI values 3000, 83.33% of cases were positive for FC-XM. Further, in those groups with MFI values between 3000 and 6000, 38.09% were positive for CDC-AHG-XM, while 86.66% showed positivity in the group with MFI >6000. Our results indicated that Luminex-DSA MFI value >1995 (P 4247 (P 5000 and >7000 for SAB assay had a sensitivity and specificity of 100% in detecting a positive IgG B-cell FC-XM and CDC-AHG-XM, respectively.
ISSN:1532-1819
1532-4230
DOI:10.1080/15321819.2020.1736095