Comparison of phenotypic methods with mecA gene based polymerase chain reaction for Methicillin-resistant Staphylococcus aureus detection

To determine methicillin resistance in staphylococcus aureus by different phenotypic methods, and to evaluate their accuracy with mecA gene polymerase chain reaction for methicillin-resistant staphylococcus aureus detection. The descriptive cross-sectional study was conducted from January to Decembe...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the Pakistan Medical Association 2020-02, Vol.70 (2), p.276-280
Main Authors: Khawaja, Aneela, Arshad, Faiqa, Khan, Idrees
Format: Article
Language:English
Subjects:
Anopheles
Anti-Bacterial Agents
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Binding proteins
Cefoxitin
Disk Diffusion Antimicrobial Tests
Genes
Genetic research
Humans
Latex Fixation Tests
Methicillin
Methicillin resistance
Methicillin Resistance - genetics
Methicillin-Resistant Staphylococcus aureus - genetics
Methicillin-Resistant Staphylococcus aureus - metabolism
Methicillin-Resistant Staphylococcus aureus - physiology
Methods
Microbial drug resistance
Oxacillin
Penicillin-Binding Proteins - genetics
Penicillin-Binding Proteins - metabolism
Penicillins
Phenotype
Polymerase Chain Reaction
Protein binding
Sensitivity and Specificity
Staphylococcal Infections - diagnosis
Staphylococcal Infections - microbiology
Staphylococcus aureus
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To determine methicillin resistance in staphylococcus aureus by different phenotypic methods, and to evaluate their accuracy with mecA gene polymerase chain reaction for methicillin-resistant staphylococcus aureus detection. The descriptive cross-sectional study was conducted from January to December 2015 at the Post- Graduate Medical Institute, Lahore, Pakistan, and comprised consecutive, non-repetitive clinical isolates of methicillin-resistant staphylococcus aureus that were screened with oxacillin disk 1μg and cefoxitin disk 30μg by Kirby-Bauer method using Clinical and Laboratory Standards Institute guideline. The isolates were cultured on oxacillin screen and mannitol salt agar, and subjected to latex agglutination for penicillin-binding protein 2aand polymerase chain reaction for mecA gene. Data was analysed using SPSS 20. All the 105 isolates were resistant on oxacillin and cefoxitin disk diffusion test, but 95(90.47%) were positive for mecA gene by latex agglutination and polymerase chain reaction. The sensitivity of oxacillin salt agar, mannitol salt agar and latex agglutination was 94.31%, 96.73% and 98.95%, respectively. Keeping polymerase chain reaction as the gold standard, the specificity and diagnostic accuracy of latex agglutination were 77.77% and 97.14% respectively, which was the highest among all the phenotypic methods. Latex agglutination method can be proposed as a swiftly reliable diagnostic technique for the detection of mecA gene in methicillin-resistant staphylococcus aureus isolates in resource-constrained settings where molecular methods are limited.
ISSN:0030-9982
DOI:10.5455/JPMA.290732