Molecular Imaging and In Situ Quantitative Profiling of Fatty Acid Synthase with a Chemical Probe

Cancer cells rely on fatty acid synthase (FASN), a key enzyme for de novo biosynthesis of long chain fatty acids, to sustain their proliferative potential and drive invasion. Unfortunately, conventional FASN assays are technically inadequate for discerning otherwise elusive FASN activity in complex...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-03, Vol.92 (6), p.4419-4426
Main Authors: Xu, Yue, Deng, Zhangshuang, Shi, Yue, Chen, Xin, Xu, Jingyuan, Zhong, Suyun, Xiao, Youli, Wong, Nai-Kei, Zhou, Yiqing
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological activity
Biosynthesis
Chemistry
Fatty Acid Synthases - analysis
Fatty Acid Synthases - metabolism
Fatty acids
Fatty-acid synthase
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Molecular Structure
Optical Imaging
Particle Size
Selectivity
Surface Properties
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Summary:Cancer cells rely on fatty acid synthase (FASN), a key enzyme for de novo biosynthesis of long chain fatty acids, to sustain their proliferative potential and drive invasion. Unfortunately, conventional FASN assays are technically inadequate for discerning otherwise elusive FASN activity in complex biological milieux, which has hindered progress in the functional study of FASN and development of its inhibitors. Here, we describe a chemical probe with unprecedented selectivity and sensitivity for the labeling of active FASN in living cells, thus demonstrating a new analytical modality for visualizing endogenous FASN activity and exploring opportunities for drug discovery.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b05327