Antibodies from Lampreys as Smart Anti-Glycan Reagents (SAGRs): Perspectives on Their Specificity, Structure, and Glyco-genomics

The repertoire of glycans expressed by individual cells and tissues is enormous, and various estimates indicate that thousands of different glycans and “glycan determinants” are critical for functional recognition by glycan-binding proteins. Defining the steady-state expression and functional impact...

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Bibliographic Details
Published in:Biochemistry (Easton) 2020-09, Vol.59 (34), p.3111-3122
Main Authors: McKitrick, Tanya R, Eris, Deniz, Mondal, Nandini, Aryal, Rajindra P, McCurley, Nathanael, Heimburg-Molinaro, Jamie, Cummings, Richard D
Format: Article
Language:English
Subjects:
Animals
Antibodies - chemistry
Antibodies - immunology
Antibody Specificity
Glycomics
Humans
Lampreys
Polysaccharides - immunology
Polysaccharides - metabolism
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Summary:The repertoire of glycans expressed by individual cells and tissues is enormous, and various estimates indicate that thousands of different glycans and “glycan determinants” are critical for functional recognition by glycan-binding proteins. Defining the steady-state expression and functional impacts of the human glycome will require a concerted worldwide effort, along with the development of new immunological, genetic, chemical, and biochemical technologies. Here, we describe the generation of smart anti-glycan reagents (SAGRs), recombinant antibodies that recognize novel glycan determinants. The antibodies are generated by the sea lamprey (Petromyzon marinus), through immunization with glycoconjugates, cells, and even tissues. SAGRs represent a versatile immunological tool for defining the expression of glycans in cells and tissues. We also present a comparison of lamprey-derived anti-carbohydrate antibodies that have been characterized to date. Finally, we explore the unique glyco-genome of the lamprey itself as it compares to those of humans and mice and how it may relate to the lamprey’s inherent capacity to produce antibodies to mammalian glycans.
ISSN:0006-2960
1520-4995
DOI:10.1021/acs.biochem.9b01015