Histopathological, genotoxic, and behavioral damages induced by manganese (II) in adult zebrafish

Manganese is a metal often found as an environmental pollutant and very associated with neurological disorders when in high concentrations. However, little is known about the effects that this contaminant can cause when in environmentally relevant concentrations and occurrence, that is, much lower t...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2020-04, Vol.244, p.125550-125550, Article 125550
Main Authors: Zimmermann Prado Rodrigues, Gabriela, Staudt, Luciane Beatris Mentges, Moreira, Marina Griebeler, dos Santos, Thainá Garbino, de Souza, Mateus Santos, Lúcio, Caio Júnior, Panizzon, Jenifer, Kayser, Juliana Machado, Simões, Leonardo Airton Ressel, Ziulkoski, Ana Luiza, Bonan, Carla Denise, de Oliveira, Diogo Losch, Gehlen, Günther
Format: Article
Language:English
Subjects:
Animals
Behavior, Animal - drug effects
Biomarkers
Chlorides
Comet Assay
DNA Damage
Ecosystem
Ecotoxicology
Liver - drug effects
Manganese - toxicity
Manganese Compounds
Micronucleus Tests
Polluting metals
Water Pollutants, Chemical - analysis
Water Pollutants, Chemical - toxicity
Zebrafish - physiology
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Summary:Manganese is a metal often found as an environmental pollutant and very associated with neurological disorders when in high concentrations. However, little is known about the effects that this contaminant can cause when in environmentally relevant concentrations and occurrence, that is, much lower than those commonly studied. So, the aim of the study was to evaluate the effects that environmentally relevant concentrations of this metal would cause in different zebrafish organs (brain, liver, and blood). Acute 96-h and chronic 30-day exposures were performed using the manganese chloride salt as a pollutant. Behavioral alterations of anxiogenic type were observed in the animals after chronic exposures to 4.0 mg L−1 MnCl2, which traveled a greater distance at the bottom of the aquarium. This may be associated with neuronal damages in the telencephalic region responsible for motor and cognitive activity of the fish, observed in animals from the same exposure. In addition, hepatic histopathological damage as vacuolization of hepatocytes and genotoxic damage, identified by comet assay and micronucleus test, was also observed after acute and chronic exposure, especially at the highest pollutant concentrations (8.0 and 16.0 mg L−1 in acute exposure, and 4.0 mg L−1 in chronic exposure. The study reinforces the risk that environmental pollutants pose to the ecosystem, even in low concentrations. •Chronic exposure to MnCl2 may cause behavioral anxiogenic type in zebrafish.•Behavioral damages may be originated from the reduction of neurons in the Vd nucleus.•MnCl2 induces micronucleus and comet in erythrocyte after acute and chronic exposures.•Liver histopathology shows vacuolization of hepatocytes after exposure to MnCl2.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2019.125550