Screening of Processed Foods for Transgenic Proteins from Genetically Engineered Plants Using Targeted Mass Spectrometry

Screening of food products for the presence of material from genetically engineered (GE) plants is typically done using deoxyribonucleic acid (DNA)-based methods to detect the presence of transgenic DNA. In this study, we have demonstrated the feasibility of using targeted mass spectrometry (MS) to...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-02, Vol.92 (4), p.3455-3462
Main Authors: Swatkoski, Stephen J, Croley, Timothy R
Format: Article
Language:English
Subjects:
3-Phosphoshikimate 1-Carboxyvinyltransferase - analysis
3-Phosphoshikimate 1-Carboxyvinyltransferase - genetics
Agrobacterium - enzymology
Agrobacterium - genetics
Cereals
Chemistry
Chromatography, Liquid
Corn
Deoxyribonucleic acid
DNA
DNA, Plant - genetics
Food
Food Analysis
Food plants
Food processing
Food production
Genetic Engineering
Glycine max - genetics
Glyphosate
Infant formulas
Mass spectrometry
Mass spectroscopy
Peptides
Plants, Genetically Modified
Processed foods
Proteins
Scientific imaging
Screening
Soybeans
Spectroscopy
Tandem Mass Spectrometry
Transgenic plants
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Summary:Screening of food products for the presence of material from genetically engineered (GE) plants is typically done using deoxyribonucleic acid (DNA)-based methods to detect the presence of transgenic DNA. In this study, we have demonstrated the feasibility of using targeted mass spectrometry (MS) to detect a protein expressed by transgenic DNA to confirm the presence of GE plant material in processed foods. Scheduled parallel reaction monitoring (sPRM) was used to detect the enzyme, 5-enolpyruvulshikimate-3-phosphate synthase, from Agrobacterium sp. strain CP4 (CP4 EPSPS), which confers glyphosate tolerance in transgenic crops. Five CP4 EPSPS surrogate peptides and their corresponding retention times identified via data-dependent LC/MS/MS analysis of a glyphosate-tolerant soybean certified reference material, GTS 40-3-2, were used to develop the sPRM assay. The assay was used to screen four soy-based infant formulas, four corn-based cereals, corn tortilla chips, and cornmeal for the presence of CP4 EPSPS. At least four of the five selected surrogate peptides were detected in nine of the products analyzed, suggesting that targeted MS can serve as a complementary analytical method to DNA-based methods for the detection of material from GE plants in processed foods.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b05577