Cantharidin suppresses gastric cancer cell migration/invasion by inhibiting the PI3K/Akt signaling pathway via CCAT1

Cantharidin (CTD) is a traditional Chinese medicine that shows an anticancer effects in multiple types of cancer cells. However, the mechanism of CTD anti-cancer function in gastric cancer (GC) is still unclear. The aim of the present study was to investigate the underlying mechanism that CTD inhibi...

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Bibliographic Details
Published in:Chemico-biological interactions 2020-02, Vol.317, p.108939-108939, Article 108939
Main Authors: Song, Mengyun, Wang, Xianfei, Luo, Yajun, Liu, Zilin, Tan, Wang, Ye, Pengcheng, Fu, Zhiming, Lu, Fei, Xiang, Wanping, Tang, Linghan, Yao, Lin, Nie, Yuqiang, Xiao, Jiangwei
Format: Article
Language:English
Subjects:
Cantharidin
Cantharidin - pharmacology
CCAT1
Cell Movement - drug effects
Down-Regulation
Gastric cancer
Gene Expression Regulation, Neoplastic - drug effects
Humans
Long non-coding RNA
Phosphatidylinositol 3-Kinases - genetics
Phosphatidylinositol 3-Kinases - metabolism
PI3K/AKt
Proto-Oncogene Proteins c-akt - genetics
Proto-Oncogene Proteins c-akt - metabolism
PTEN Phosphohydrolase - genetics
PTEN Phosphohydrolase - metabolism
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
Signal Transduction - drug effects
Stomach Neoplasms
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Summary:Cantharidin (CTD) is a traditional Chinese medicine that shows an anticancer effects in multiple types of cancer cells. However, the mechanism of CTD anti-cancer function in gastric cancer (GC) is still unclear. The aim of the present study was to investigate the underlying mechanism that CTD inhibits proliferation and migration through suppression of the PI3K/Akt signaling. CTD induced GC cell apoptosis and inhibited metastasis measured by CCK8 assays as well as wound healing assays and transwell assays. Mechanistic investigations suggested that CTD modulated the PI3K/Akt signaling via western-blot and quantitative q-PCR. In addition, we identified and confirmed CCAT1 as a novel direct target of CTD inhibited PI3K/AKt signaling expression. In conclusion, our results provide new point into the critical role of CTD in suppressing PI3K/Akt signaling via down-regulation of CCAT1, resulting in suppression GC cell growth and migration/invasion. •Cantharidin suppressed MGC803 and BGC823 cell metastasis by inhibiting PI3K/Akt signal pathway in vitro.•Down-regulated CCAT1 by cantharidin may inhibit gastric cancer metastasis.•CCAT1 may regulate PI3K/Akt signal pathway in gastric cancer cell.
ISSN:0009-2797
1872-7786
DOI:10.1016/j.cbi.2020.108939